Pharmacological correction of metabolic disturbances in experimental acute pancreatitis on the background of chronic alcohol intoxication
Background: Acute pancreatitis ranks third among all acute surgical diseases of the abdominal cavity and is one of the important unresolved problems in emergency surgery. The aim of the study: to establish the features of immunometabolic changes in acute pancreatitis with short-term and chronic ethanol intoxication, to develop ways of pharmacological correction of the revealed disorders. Materials and methods: The studies were conducted on 377 healthy sexually mature Wistar rats. Alcoholic intoxication was modeled by intragastric injection of 20% ethanol solution at a dose of 3 ml/kg after 24 hours for 5 days. In chronic alcohol intoxication (CAI) ethanol was injected for 30 or 60 days (CAI-30, CAI-60). Acute pancreatitis was modeled (R.N. Wang) in the modification of A.S. Alekhin (2006) by ligating the duct of the left and right lobes of the pancreas and stimulating the prozerin three times every 60 min. Results: Short-term intoxication (5 days) with ethanol causes minor metabolic changes of reactive nature, 30-day, more than 60-day injection, leads to the development of toxic liver damage, oxidative stress, imbalance in the formation of adaptive and innate immune response, disorders of functional and metabolic activity of circulating red blood cells. In the conditions of acute destructive pancreatitis (ADP) on the background of 30-day alcohol intoxication 97.1% of the laboratory parameters were violated. Injection of Phosphogliv, Hypoxene and Hepon normalized 12.1% of altered metabolic parameters, corrected – 60.6% and did not affect – 27.3%. The combination of Heptral, Glutoxim and Mexidol was more effective because it corrected 42.4% and normalized 57.8% of the studied parameters of immunometabolic status. In the conditions of ethanol intoxication within 60 days in acute pancreatitis, 100% of laboratory parameters were violated. Injection of Phosphogliv, Hypoxene and Hepon corrected 38.2% and normalized 11.8%. The combination of Heptral, Glutoxim and Mexidol corrected 67.6% and normalized 17.6% of the studied parameters. Conclusion: The combination of Heptral, Glutoxim and Mexidol (84 and 37 points, respectively) was more preferable than Phosphogliv, Hypoxene and Hepon (46 and 15 points, respectively) in the assessment of their own corrective effects in EDP on the background of 30 - and 60-day alcohol intoxication.