Comparative evaluation of the effectiveness of DMAE derivative 7-16, carbamilated darbepoetin and substance C7070 in correction of hypertensive neuroretinopathy in rats
Background: Increasing the effectiveness of drug therapy for hyperintensive neuroretinopathy is one of the important tasks of pharmacology and ophthalmology. The aim of the study:To assess the effectiveness of DMAE derivative 7-16, carbamylated darbepoetin and C7070 in comparative aspect in correction of hypertensive neuroretinopathy in rats. Materials and methods: The level of retinal microcirculation was assessed by laser Doppler flowmetry (LDF) using the Biopac-systems MP-150 software and hardware complex and the needle type sensor TSD-144 (USA), and the program AcqKnowledge 4.2. Dimethylaminoethanol derivative DMAE 7-16 (OJS company "All-Russian Scientific Center for safety of biologically active substances", Russia) was injected in doses of 12.5 mg/kg/day, 25 mg/kg/day intragastric. Substance C7070 (3-(1H-benzimidazole-2-yl)-1,2,2-trimethylcyclopentanone acid) (JSC "Experimental Plant "VLADMIVA", Russia) was injected intragastrically in a dose of 50 mg/kg. Carbamylated darbepoetin was injected subcutaneously in a dose of 300 mcg/kg. Picamilon (Pharmstandard-Ufavita, Russia) was chosen as a comparison drug injected from 22nd to 28th day of the experiment inclusively, intragastrically, in a dose of 30 mg/kg. The evaluation of the clinical course of hypertensive neuroretinopathy with the correction by investigated pharmacological agents was performed on day 29th of the experiment. Results: Effective retinal microcirculation correction was found in DMAE derivative 7-16 in a dose of 25 mg/kg and C7070 in a dose of 50 mg/kg, which exceeds the mean value in the group with picamilon correction and is comparable to the mean value in the control group. In groups with DMAE derivative 7-16 in a dose of 12.5 mg/kg and carbamylated darbepoetin in a dose of 300 µg/kg, the target values of the microcirculation level in retina were not achieved. According to the results of evaluation of the clinical course of hypertensive neuroretinopathy in the groups with the introduction of substance C7070, carbamylated darbepoetin and picamilon, the parameters of the eye fundus were returned to normal. With the introduction of DMAE derivative 7-16 in a dose of 12.5 mg/kg there was swelling and partial optic disc decoloration; in a dose of 25 mg/kg there was no edema. Conclusion: The most effective correction of hypertensive neuroretinopathy in Wistar rats was detected in DMAE derivative 7-16 in a dose of 25 mg/kg and C7070 in a dose of 50 mg/kg, superior to the picamilon.