Analysis of the association between C936T VEGFA gene polymorphism and diabetic foot syndrome in the Ukrainian population
Background: The dysfunction of vascular endothelial growth factor A (VEGFA) is one of the leading factors of macro- and microangiopathy development in diabetic foot syndrome (DFS) patients. Recently, a number of experimental studies in various populations have been carried out to test the association between VEGFA gene polymorphisms and development of chronic hyperglycemia vascular complications. At the same time, there are no such studies in Ukrainian population. The aim of the study: The aim of this work was to check the possible association between C936T VEGFA gene polymorphism and DFS development in Ukrainian patients with type 2 diabetes mellitus (T2DM). Materials and methods: Venous blood of 154 patients with T2DM complicated by DFS and 124 individuals without diabetes and glucose intolerance was used. The polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP) was performed for VEGFA C936T (rs3025039) polymorphism genotyping. SPSS-17 was used for most statistical analyses. Results: It was found that ratio of C/C-homozygotes, C/T-heterozygotes and T/T-homozygotes (C936T VEGFA gene polymorphism) in patients with DFS was 47.4%, 41.6% and 11.0%; in the control group – 50.0%, 43.5%, 6.5%, respectively. Comparison of these genotypes frequencies between DFS patients and control subjects using χ2-Pearson criterion showed no significant difference (P = 0.413). The results of regression analysis under the dominant, recessive, superdominant and additive inheritance models also revealed no association between VEGFA C936T genotypes and DFS development (P>0.05). Conclusion: There is no link between VEGFA C936T polymorphism and risk of DFS development in Ukrainian patients with T2DM.
Rusanov AV, Chumachenko YaD, Dubovyk YI, et al. Analysis of the association between C936T VEGFA gene polymorphism and diabetic foot syndrome in the Ukrainian population. Research Results in Biomedicine. 2019;5(2):34-43. (In Rus-sian) DOI: 10.18413/2658-6533-2019-5-2-0-1
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The work is an integral part of the research theme «Molecular genetic and morphological features of the regeneration of lower limb tissues under chronic hyperglycemia» (state registration number 0117U003926)