The study of neurodynamic disturbances in rats with cranial injury
Background: Domestic and occupational injuries, including traumatic brain injury (TBI), are diseases that are a major public health problem in all industrialized countries and result in incapacitation, as well as high mortality, disability and lead to high costs of treatment. The aim of the study: Search for an adequate experimental pathology model for evaluating the effectiveness of pharmacological correction of traumatic brain injury in rats. Materials and methods: The study was carried out on 40 adult male rats of the “Wistar” line, which were used to simulate a craniocerebral injury using loads of different weights. Next, we evaluated the neurological deficit in the dynamics and performed a morphological study of sections of the brain of animals. Results: With an increase in the load mass in the experiment, the behavioral activity of rats decreased. This was manifested in a decrease in overall activity, the number of stereotypical movements, maximum speed of movement, and total distance. Rest time increased. The data obtained in the behavioral tests “Rota-rod” and “Infrared activity monitor” confirm the severity of TBI in the experimental groups. Based on the mortality rate and severity of TBI, the histological picture in the priority group (m = 155 g) is similar to the morphological picture in humans with this kind of injury. Conclusion: The parameters of this model (with load weight 155 grams, drop height 0.6 meters, and frontotoparietal impact area) make it possible to obtain a variety of neurological symptoms, which can be subjected to quantitative and qualitative assessment, including dynamics. The results of structural changes in the brain tissue correlate with the data of neurological deficit and behavioral status of laboratory animals (structural changes in the brain tissue are preserved up to the 7th day against the background of signs of reparative processes).