Polymorphism of the VEGFA gene, smoking and coronary heart disease: the significance of gene-environmental interactions for disease susceptibility
Background: Coronary heart disease (CHD) is a common disease with a high mortality rate in Russia, so the problem of early prognosis and prevention of this disease is particularly relevant. In connection with the multifactorial etiology of CHD, understanding the genetic factors of the disease development for medical professionals is promising for more complete formation of risk groups among the population of our country and carrying out preventive measures. The aim of the study: To investigate the role of interactions between VEGFA gene polymorphisms and smoking in coronary heart disease (CHD) risk. Materials and methods: DNA samples from 1214 unrelated individuals (555 CHD patients and 659 controls) were genotyped for SNPs rs3025039, rs2010963, rs833061, rs3025000 and rs833068 of VEGFA by the TaqMan-based assays. Results: In this study, we found that the alleles rs2010963C, rs833061T, rs3025000T, and rs833068A were associated with increased expression of the VEGFA gene in body tissues. For the first time we have identified associations of the polymorphic variants of rs2010963, rs833061, rs3025000 and rs833068 with the risk of developing coronary heart disease exclusively in smokers, regardless of gender and age (PGxE£0,01). Conclusion: In the framework of this work, SNPs rs3025000 and rs833068 were first presented by us as genetic markers of predisposition to coronary heart disease. The considered polymorphic variants of the VEGFA gene (rs2010963, rs833061, rs3025000 and rs833068) are associated with the risk of developing heart artery disease by potentiating the negative effects of the chemical components of smoke on the formation of the atherosclerotic process in the coronary arteries.