2658-6533Research Results in Biomedicine2658-653310.18413/2313-8955-2018-4-1-70-761376Archive categoriesSTUDY OF POLYSACCHARIDES OF THE ACROPTILON REPENS L. HERB OF THE FLORA OF TULA REGIONSTUDY OF POLYSACCHARIDES OF THE ACROPTILON REPENS L. HERB OF THE FLORA OF TULA REGIONShestopalovaNatalia N.ShestopalovaNatalia N.shestopalova@bsu.edu.ru20184100

The aim of the research was to study the component composition of polysaccharide complexes of the Acroptilon repens L. herb. Materials and methods. The air-dry Acroptilon repens L. herb, harvested in 2017 on the territory of the Tula region in the period of mass flowering was used as an object of the research. Isolation of polysaccharide complexes was carried out fractionally, according to the method of Kochetkov (first, there were obtained water-soluble polysaccharide complexes, and then – pectin substances and hemicelluloses A and B). The monosaccharide composition of the isolated polysaccharide complexes was established by acid hydrolysis, followed by chromatography with n-butanol – pyridine – water solvent systems purified (6 : 4 : 3) and ethylacetate – acetic acid – formic acid – purified water (18 : 3 : 1 : 4 ) in parallel with reliable samples. The quantitative content of sugars in the hydrolysates of polysaccharides was determined by densitometry. Titrometric methods were used for the quantitative determination of functional groups in the pectin substances of the investigated raw material. Results of the study. In the course of the study, water-soluble polysaccharide complexes, pectin substances, hemicelluloses A and B were isolated from the Acroptilon repens L. herb. It was established that pectic substances (12.6%) and water-soluble polysaccharides (4.03%) were the predominant substances. In water-soluble polysaccharides of the Acroptilon repens L. herb, arabinose (7.7%) and galactose (6.9%) predominate. The basis of pectin substances is galacturonic acid (83.6%). In hemicelluloses, xylose is the predominant monosaccharide. The pectin substances of the Acroptilon repens L. herb are characterized by a low (<50%) degree of esterification.

The aim of the research was to study the component composition of polysaccharide complexes of the Acroptilon repens L. herb. Materials and methods. The air-dry Acroptilon repens L. herb, harvested in 2017 on the territory of the Tula region in the period of mass flowering was used as an object of the research. Isolation of polysaccharide complexes was carried out fractionally, according to the method of Kochetkov (first, there were obtained water-soluble polysaccharide complexes, and then – pectin substances and hemicelluloses A and B). The monosaccharide composition of the isolated polysaccharide complexes was established by acid hydrolysis, followed by chromatography with n-butanol – pyridine – water solvent systems purified (6 : 4 : 3) and ethylacetate – acetic acid – formic acid – purified water (18 : 3 : 1 : 4 ) in parallel with reliable samples. The quantitative content of sugars in the hydrolysates of polysaccharides was determined by densitometry. Titrometric methods were used for the quantitative determination of functional groups in the pectin substances of the investigated raw material. Results of the study. In the course of the study, water-soluble polysaccharide complexes, pectin substances, hemicelluloses A and B were isolated from the Acroptilon repens L. herb. It was established that pectic substances (12.6%) and water-soluble polysaccharides (4.03%) were the predominant substances. In water-soluble polysaccharides of the Acroptilon repens L. herb, arabinose (7.7%) and galactose (6.9%) predominate. The basis of pectin substances is galacturonic acid (83.6%). In hemicelluloses, xylose is the predominant monosaccharide. The pectin substances of the Acroptilon repens L. herb are characterized by a low (<50%) degree of esterification.

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