Background: Acute kidney injury is an urgent health problem at the junction of specialties with a high level of disability and mortality of patients. The pertinence of the study stems from conflicting data on the potential mechanisms for preventing this type of injury and the lack of a single therapeutic strategy, despite the possibility of substitution therapy. The aim of the study: To study the possibility of correcting ischemic and reperfusion renal injuries in an experiment with asialo erythropoietin and a selective inhibitor of arginase II KUD975. Materials and methods: In a series of experiments on Wistar male rats, there were studied the renoprotective properties of the prophylactic application of the combination of asialo erythropoietin (2.4 μg/kg 30 minutes before the induction of ischemia) and the selective inhibitor of arginase II KUD975 (120 mg/kg 120 minutes before the induction of ischemia) on a 40-minute bilateral model of renal ischemia-reperfusion. Renoprotective properties were evaluated by the results of biochemical markers of acute renal damage, the dynamics of glomerular filtration rate and fractionated sodium excretion, as well as the severity of microcirculatory disorders. Results: It has been established that the prophylactic use of the combination of asialo erythropoietin KUD975 leads to a decrease in the serum concentration of markers of acute renal damage, an increase in the glomerular filtration rate, a decrease in fractional sodium excretion, and a decrease in microcirculatory disorders. Conclusion: Correction of ischemic and reperfusion renal injuries in the experiment with asialo erythropoietin and selective inhibitor of arginase II KUD975 is an effective strategy for the prevention and treatment of acute kidney injury.