2658-6533Research Results in Biomedicine2658-653310.18413/2313-8955-2018-4-4-0-81600Medicine (miscellaneous)The value of determining concentrations of calcidiol and vascular endothelial growth factor to predict non-developing pregnancyThe value of determining concentrations of calcidiol and vascular endothelial growth factor to predict non-developing pregnancyEnkovaElena V.EnkovaElena V.enkova@bk.ruKhoperskayaОlga V.KhoperskayaОlga V.smv250587@mail.ru20184400

Background: Non-developing pregnancy is one of the most difficult medical and social problems of modern obstetrics and gynecology, which has no tendency to decrease. Previously occurred non-developing pregnancy is a predictor of adverse outcomes of subsequent pregnancies, up to the usual miscarriage. Non-developing pregnancy is a multifactorial and polyetiological complication of gestation, which necessitates a comprehensive assessment of the probability of a pathological condition in each case. The aim of the study: Assessment of factors contributing to the formation of non-developing pregnancy to identify patients related to a risk group. Determining the status of vitamin D and plasma serum concentration of VEGF in patients with non-developing pregnancy as informative markers for predicting a pathological condition. Materials and methods: The study involved 116 patients: 76 patients with non-developing pregnancy and 40 patients with physiological pregnancy. The information about reproductive and somatic health of women, their social status was collected. The levels of neuroticism and anxiety were explored using the scale of Hake and Hess and the scale of Ch. D. Spielberg and Yu. L. Khanin. The types of accentuation of temperament were identified based on the G. Smishek and K. Leongard test results. The level of perinatal risk was determined using the scale of V. E. Radzinsky, S. A. Knyazev, I. N. Kostin (2011). The enzyme immunoassay 25-OH Vitamin D blood plasma was performed using a set of reagents ELISA, EUROIMMUN AG (Germany), VEGF - human VEGF-A ELISA using reagents from Thermo Fisher Scientific (USA). Results: The factors contributing to the development of the studied pathological condition were determined. Vitamin D deficiency has been found to be a risk factor for non-developing pregnancy. With a decrease of calcidiol concentration below 10 ng / ml in the plasma, the probability of developing a non-developing pregnancy increases by 4.74 times. The level of VEGF in patients with non-developing pregnancy is 5.1 times higher than in the control group. Conclusion: Determining the status of vitamin D and the concentration of VEGF is an additional diagnostic method to predict the likelihood of non-developing pregnancy. Identification of patients risking the development of non-developing pregnancy is possible with the help of a thorough history collection, clarification of social factors contributing to the emergence of a pathological condition and evaluation of psychoemotional status. For timely correction of hypovitaminosis, patients with a high risk of developing the studied complication of gestation are recommended to have their level of plasma calcidiol tested.

Background: Non-developing pregnancy is one of the most difficult medical and social problems of modern obstetrics and gynecology, which has no tendency to decrease. Previously occurred non-developing pregnancy is a predictor of adverse outcomes of subsequent pregnancies, up to the usual miscarriage. Non-developing pregnancy is a multifactorial and polyetiological complication of gestation, which necessitates a comprehensive assessment of the probability of a pathological condition in each case. The aim of the study: Assessment of factors contributing to the formation of non-developing pregnancy to identify patients related to a risk group. Determining the status of vitamin D and plasma serum concentration of VEGF in patients with non-developing pregnancy as informative markers for predicting a pathological condition. Materials and methods: The study involved 116 patients: 76 patients with non-developing pregnancy and 40 patients with physiological pregnancy. The information about reproductive and somatic health of women, their social status was collected. The levels of neuroticism and anxiety were explored using the scale of Hake and Hess and the scale of Ch. D. Spielberg and Yu. L. Khanin. The types of accentuation of temperament were identified based on the G. Smishek and K. Leongard test results. The level of perinatal risk was determined using the scale of V. E. Radzinsky, S. A. Knyazev, I. N. Kostin (2011). The enzyme immunoassay 25-OH Vitamin D blood plasma was performed using a set of reagents ELISA, EUROIMMUN AG (Germany), VEGF - human VEGF-A ELISA using reagents from Thermo Fisher Scientific (USA). Results: The factors contributing to the development of the studied pathological condition were determined. Vitamin D deficiency has been found to be a risk factor for non-developing pregnancy. With a decrease of calcidiol concentration below 10 ng / ml in the plasma, the probability of developing a non-developing pregnancy increases by 4.74 times. The level of VEGF in patients with non-developing pregnancy is 5.1 times higher than in the control group. Conclusion: Determining the status of vitamin D and the concentration of VEGF is an additional diagnostic method to predict the likelihood of non-developing pregnancy. Identification of patients risking the development of non-developing pregnancy is possible with the help of a thorough history collection, clarification of social factors contributing to the emergence of a pathological condition and evaluation of psychoemotional status. For timely correction of hypovitaminosis, patients with a high risk of developing the studied complication of gestation are recommended to have their level of plasma calcidiol tested.

non-developing pregnancyvitamin DVEGFcalcidiolangiogenesisnon-developing pregnancyvitamin DVEGFcalcidiolangiogenesisСписок литературыOlina AA, Sadykova GK, Galinova IV. The structure of reproductive losses. Perm medical journal. 2017;34(6):59-66. Russian. DOI: 10.17816/pmj34659%66Radzinsky VE, Orazmuradova AA, editors. [Early pregnancy. From pregravid preparation for a healthy pregnancy]. Mоscow: Editorial Board StatusPraesens; 2018. 800 p. Russian.Ota К, Dambaeva S, Kim MW, et al. 1.25-Dihydroxy-vitamin D3 regulations NK-cell cytotoxicity, cytokine secretion, and degranulation in women with recurrent pregnancy. Europ. J. of immunology. 2015;45(11):3188-3199. DOI: 10.1002/eji.201545541Grundmann M, Haidar M, Placzko S, et al. Vitamin D improves the angiogenic properties of endothelial progenitor cells. Am. J. Physiol. Cell Physiol. 2012;303:954-962. DOI: https://doi.org/10.1152/ajpcell.00030.2012 W, Yan XT, Bai CM, et al. Decreased serum vitamin D levels in early spontaneous pregnancy loss. Eur. J. Clin. Nutr. 2016;70(9):1004-8. DOI: 10.1038/ejcn.2016.83Lerchbaum E, Rabe Т. Vitamin D and female fertility. Curr. Opin. Obstet. Gynecol. 2014;26(3):145-150. DOI: 10.1097/GCO.0000000000000065Achkar M,Dodds L, Gigue`re Y, et al. Vitamin D status in early pregnancy and risk of preeclampsia. Am. J. Obstet. Gynecol. 2015;212(4):511. DOI: https://doi.org/10.1016/j.ajog.2014.11.009Voropaeva EE, Kazachkov EL, Medvedev BI, et al. [Self-induced abortion: monograph]. Chelyabinsk: Izdatel'stvo Yuzhno-Ural'skogo gosudarstvennogo meditsinskogo universiteta; 2014. 163 p. Russian.Sidelnikova VM. [Preparation and management of pregnancy in women with habitual miscarriage: method. manuals and clinical protocols]. Moscow: Medpress-inform; 2013. 249 p. Russian.Simon L, Zini A, Dyachenko A, et al. A systematic review and meta-analysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic outcome. Asian J. of Andrology. 2017;19(1):80-90.Iversen ML, Sørensen NO, Broberg L, et al. Alcohol consumption and binge drinking in early pregnancy. A cross-sectional study with data from the Copenhagen Pregnancy Cohort. BMC Pregnancy Childbirth. 2015;15:327. DOI: 10.1186/s12884-015-0757-zBastos R, Ramalho C, Dória S. Prevalence of chromosomal abnormalities in spontaneous abortions or fetal deaths. Acta. Med. Port. 2014;27(1):42-8.Dudenkova MR, Gorbunov ES. Analysis of medico-social characteristics and pharmacotherapy of women with a diagnosis of non-developing pregnancy. Medical journal of Bashkortostan. 2018;13(2):64-67. Russian.Ligidova AT. [Pathogenetic aspects of threatened abortion in the 1st trimester] [dissertation]. Rostov-on-don; 2011. Russian.Radzinsky VE, Makletsova SA, Aliyev IA, et al. [Undeveloping pregnancy: method. Recommendations]. Moscow: Statusreasens; 2015. 48 p. Russian.Dobrokhotova JЕ, Gankovskaya LV, Bakhareva IV, et al. The role of immune mechanisms in the pathogenesis of pregnancy and miscarriage. Obstetrics and gynecology. 2016;7:5-1071. Russian. DOI: https://dx.doi.org/10.18565/aig.2016.7.5-10Shelepova ES. [Features of vitamin D metabolism in preeclampsia] [dissertation]. St. Petersburg; 2018. Russian.Zhao X, Fang R, Yu R, et al. Maternal Vitamin D Status in the Late Second Trimester and the Risk of Severe Preeclampsia in Southeastern China. Nutrients. 2017;9(2):138-153. DOI: 10.4172/2155-9600.C1.021Dedov II, Melnichenko G. [Vitamin D Deficiency in adults: diagnosis, treatment, prevention: clinical recommendations]. Moscow: Endokrinologicheskiy nauchnyy tsentr; 2015. 75p. Russian.Tretyakova TB, Bashmakova NI, Demchenko NW. The Role of polymorphic gene variants of vascular endothelial growth factor VEGF-A in the formation of undeveloping pregnancy. Problems of reproduction. 2016;22(6):33-37. Russian. DOI:10.17116/repro201622633-37Kim M,Park HJ, Seol JW, et al. VEGF-A regulated by progesterone governs uterine angiogenesis and vascular remodelling during pregnancy. EMBO Mol. Med. 2013;5(9):1415-1430. DOI: 10.1002/emmm.201302618