2658-6533Research Results in Biomedicine2658-653310.18413/2658-6533-2020-6-1-0-91966Medicine (miscellaneous)<strong>Features of the species composition and functional activity of mast cells in the decidual tissue of patients with undeveloped pregnancy and </strong><strong>polycystic ovary syndrome</strong><br /> &nbsp;<strong>Features of the species composition and functional activity of mast cells in the decidual tissue of patients with undeveloped pregnancy and </strong><strong>polycystic ovary syndrome</strong><br /> &nbsp;EnkovaValeria V.EnkovaValeria V.enkova_lera@mail.ruKhoperskayaОlga V.KhoperskayaОlga V.smv250587@mail.ruEnkovaElena V.EnkovaElena V.enkova@bk.ruOlinaAnna A.OlinaAnna A.olina29@mail.ruNovikovaLyubov A.NovikovaLyubov A.muzkvkd@mail.ru20206100

Background: Infertility, miscarriage, birth of full-term healthy children take dominant positions among the problems of obstetrics and gynecology. Рolycystic ovary syndrome is a disease one of the clinical manifestations of which is infertility, severe complications of gestation оf spontaneous pregnancy in metabolically uncompensated patients, which makes a significant contribution to the structure of perinatal morbidity, and sometimes mortality. The aim of the study: The aim of the study was to study the species composition and functional activity of mast cells in decidual tissue of patients with рolycystic ovary syndrome and missed abortion, to compare the data obtained with those from patients with missed abortion without metabolic disorders and during the physiological course of pregnancy. Materials and methods: A total of 60 patients were examined. Immunohistochemical identification of mast cell tryptase and chymase in decidual tissue samples of patients was performed using mouse monoclonal antibodies to tryptase and chymase. Secondary antibodies for tryptase (Goat Anti-Rabbit Ig H&amp;L conjugated with Alexa Fluor-488) and chemase (Goat-Anti-Mouse Ig H&amp;L conjugated with Cy3) were used to visualize proteases in multiple immunomarking. Results: The number of mast cells in the decidual tissue of patients with missed abortion and POS is 4,4 times higher than in physiological pregnancy and 1,38 times higher in undeveloped pregnancy and the absence of metabolic disorders. The protease profile of mast cells is shifted towards chemase expression. The comparable functional activity of the studied cells in missed abortion in both groups indicates the absence of the influence of рolycystic ovary syndrome on the degree of mast cell degranulation. Conclusion: The changes in the quantitative and qualitative composition of mast cells confirms their participation in the pathogenetic mechanisms of the formation of missed abortion on a background оf metabolic disorders typical for рolycystic ovary syndrome.

Background: Infertility, miscarriage, birth of full-term healthy children take dominant positions among the problems of obstetrics and gynecology. Рolycystic ovary syndrome is a disease one of the clinical manifestations of which is infertility, severe complications of gestation оf spontaneous pregnancy in metabolically uncompensated patients, which makes a significant contribution to the structure of perinatal morbidity, and sometimes mortality. The aim of the study: The aim of the study was to study the species composition and functional activity of mast cells in decidual tissue of patients with рolycystic ovary syndrome and missed abortion, to compare the data obtained with those from patients with missed abortion without metabolic disorders and during the physiological course of pregnancy. Materials and methods: A total of 60 patients were examined. Immunohistochemical identification of mast cell tryptase and chymase in decidual tissue samples of patients was performed using mouse monoclonal antibodies to tryptase and chymase. Secondary antibodies for tryptase (Goat Anti-Rabbit Ig H&amp;L conjugated with Alexa Fluor-488) and chemase (Goat-Anti-Mouse Ig H&amp;L conjugated with Cy3) were used to visualize proteases in multiple immunomarking. Results: The number of mast cells in the decidual tissue of patients with missed abortion and POS is 4,4 times higher than in physiological pregnancy and 1,38 times higher in undeveloped pregnancy and the absence of metabolic disorders. The protease profile of mast cells is shifted towards chemase expression. The comparable functional activity of the studied cells in missed abortion in both groups indicates the absence of the influence of рolycystic ovary syndrome on the degree of mast cell degranulation. Conclusion: The changes in the quantitative and qualitative composition of mast cells confirms their participation in the pathogenetic mechanisms of the formation of missed abortion on a background оf metabolic disorders typical for рolycystic ovary syndrome.

рolycystic ovary syndromemast cellsdecidual tissuemissed abortionрolycystic ovary syndromemast cellsdecidual tissuemissed abortionСписок литературыMiklyaeva IA, Danilova IK. [Topical issues of polycystic ovary syndrome in women of reproductive age]. Molodoy uchenyy. 2018;24:285-289. Russian.&nbsp;Adamyan LV, Andreeva EN, Gasparyan SV, et al. editors. Clinical recommendations: polycystic ovarian syndrome in reproductive age (treatment protocol). Moscow; 2015. Russian.&nbsp;Yildiz BO,&nbsp;Bozdag G,&nbsp;Yapici Z, et al. Prevalence, phenotype and cardiometabolic risk of polycystic ovary syndrome under different diagnostic criteria. J. Hum. Reprod. 2012;27:67-73. DOI: 10.1093/humrep/des232&nbsp;Pustotina OA, Akhmedova EA. Pregravid preparation of women with a history of miscarriage. Medical Council. 2016;4:130-136. Russian. DOI: 10.21518/2079-701X-2016-4-130-136&nbsp;Cavalcante MB,&nbsp;Sarno M,&nbsp;Cavalcante CT, et al. Coagulation Biomarkers in Women with Recurrent Miscarriage and Polycystic Ovarian Syndrome: Systematic Review and Meta-Analysis. Geburtshilfe Frauenheilkd. 2019;79(7):697-704. DOI:&nbsp;10.1055/a-0884-3212&nbsp;Lindsey AS, Sunni LM,&nbsp;Daniel LK, et al. Association of testosterone and antim&uuml;llerian hormone with time to pregnancy and pregnancy loss in fecund women attempting pregnancy. Fertil. Steril. 2018;109(3):540-548. DOI:&nbsp;10.1016/j.fertnstert.2017.11.014Atyakshin DA, Burtseva AS, Alekseeva NT. Tryptase as a multifunctional component of mast cell secretion. Journal of anatomy and histopathology. 2017;6(1):121-132. Russian.Chang EM,&nbsp;Han JE,&nbsp;Seok HH, et al. Insulin resistance does not affect early embryo development but lowers implantation rate in in vitro maturation-in vitro fertilization-embryo transfer cycle. Clin. Endocrinol. 2013;79(1):93-99. DOI: 10.1111/cen.12099Fauser BC,&nbsp;Tarlatzis BC,&nbsp;Rebar RW, et al. Consensus on womens health aspects of polycystic ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop. Group. Fertil Steril. 2012;97(1):28-38. DOI: 10.1016/j.fertnstert.2011.09.024Sterling L, Liu J, Okun N, et al. Pregnancy outcomes in women with polycystic ovary syndrome undergoing in vitro fertilization. Fertil. Steril. 2016;105(3):91-97. DOI:&nbsp;10.1016/j.fertnstert.2015.11.019Runqin L, Juan W,&nbsp;Junlin H, et al. Mice endometrium receptivity in early pregnancy is impaired by maternal hyperinsulinemia. Mol. Med. Rep. 2017;15(5):2503-2510. DOI:&nbsp;10.3892/mmr.2017.6322Yakovlev PP, Kogan IY. [Endometrium and polycystic ovary syndrome]. Journal of obstetrics and women&#39;s diseases. 2018;67(4):60-66. Russian. DOI: 10.17816/JOWD67460-66Younas K, Quintela M, Thomas S, et al. Delayed endometrial decidualisation in polycystic ovary syndrome. the role of AR-MAGEA11. J. Mol. Med. (Berl). 2019;97(9):1315-1327. DOI:&nbsp;10.1007/s00109-019-01809-6Piltonen TT,&nbsp;Chen JC,&nbsp;Khatun M, et al. Endometrial stromal fibroblasts from women with polycystic ovary syndrome have impaired progesterone-mediated decidualization, aberrant cytokine profiles and promote enhanced immune cell migration in vitro. Hum. Reprod. 2015;30(5):1203-1215. DOI: 10.1093/humrep/dev055K,&nbsp;Meyer N,&nbsp;Schumacher A, et al. Transfer of regulatory T cells into abortion-prone mice promotes the expansion of uterine mast cells and normalizes early pregnancy angiogenesis. Scientific Reports. 2015;5:13938. DOI: 10.1038/srep13938Atiakshin D, Buchwalow I, Samoilova V, et al. Characterization of mast cell populations using different methods for their identification. Histochemistry and Cell Biology. 2017;147(6):683-694.&nbsp;Doi: 10.1007/s00418-017-1547-7. Russian.Buchwalow IB, Boecker W. Immunohistochemistry: basics and methods. Springer; 2010.Radzinsky VE, Orazmuradov AA. [Pregnancy of early terms. From pregravid preparation to healthy gestation]. Moscow: Redaktsiya zhurnala StatusPraesens; 2018. Russian.Gaiskaya OV. [Clinical and diagnostic significance of specific markers in threatened miscarriage in pregnant women in the first trimester] [dissertation]. Voronezh (Russia): Voronezh state medical University named after N.N. Burdenko; 2018. Russian.Khoperskaya O. [Clinical, laboratory, and pathologic features of non-developing pregnancy] [dissertation]. Voronezh (Russia): Voronezh state medical University named after N.N. Burdenko; 2018. Russian.