2658-6533Research Results in Biomedicine2658-653310.18413/2658-6533-2023-9-1-0-52985Pharmacology<strong>State of colon parietal microbiota and antioxidant properties of colonocytes in rats with ecological dysbiosis treated with sea vitamin E and buckthorn oil</strong><br /> &nbsp;<strong>State of colon parietal microbiota and antioxidant properties of colonocytes in rats with ecological dysbiosis treated with sea vitamin E and buckthorn oil</strong><br /> &nbsp;KorolevVladimir A.KorolevVladimir A.medecol1@yandex.ruMedvedevaOlga A.MedvedevaOlga A.olgafrida@rambler.ruRiadnovaVera A.RiadnovaVera A.veraan8@ya.ruShevchenkoAlina V.ShevchenkoAlina V.alina7227@mail.ruShekhovtsovaOksana V.ShekhovtsovaOksana V.shechovcova@mail.ruKorolevIvan V.KorolevIvan V.korolevva@kursksmu.netKorolevEgor V.KorolevEgor V.korolevva@kursksmu.net20239100

Background:&nbsp;When the macroorganism is exposed to pesticides, qualitative and/or quantitative changes in the microbiocenosis composition occur &ndash; dysbiosis. As a result of this action, lipid peroxidation is enhanced and work of the body&#39;s antioxidant system is disrupted. The aim of the study:&nbsp;To study the state of colon microbiota and antioxidant properties of colonocytes in rats with subchronic thiram intoxication treated with vitamin E and sea buckthorn oil. Materials and methods:&nbsp;Animals in the experiment received thiram. Vitamin E and sea buckthorn oil were used as antioxidants. The composition of rat colon mucosal microflora was judged. The activity of antioxidant protection enzymes of colonocytes was studied by content of superoxide dismutase and catalase. The state of lipid peroxidation was assessed by content of diene conjugates and malondialdehyde. Results:&nbsp;The administration of thiram led to a decrease in the number of Bifidobacteria by 3 times (p&lt;0.001), Lactobacillus by 2.5 times (p&lt;0.001), E. coli with normal enzymatic activity by 2.2 times (p&lt;0.001), Escherichia with reduced enzymatic activity by 2.8 times (p&lt;0.001), Enterobacter by 1.8 times (p&lt;0.001), Salmonella by 1.7 times (p&lt;0.01), Citrobacter by 1.75 times (p&lt;0.01), coagulase-negative Staphylococci by 2.1 times (p&lt;0.001), Enterococci by 1.5 times (p&lt;0.05). There was a decrease in the activity of catalase by 1.9 times (p&lt;0.001), superoxide dismutase by 2.3 times (p&lt;0.001) and an increase in the concentration of malondialdehyde by 2.6 times (p&lt;0.001), diene conjugates by 2.4 times (p&lt;0.001). The use of vitamin E contributed to an increase in the number of Lactobacillus by 1.4 times (p&lt;0.05), a decrease in the number of Enterobacter, Morganella and Acinetobacter by 1.5 times (p&lt;0.05), 2 times (p&lt;0.001) and 1.8 times (p&lt;0.01), respectively. A decrease in the content of malondialdehyde by 3 times (p&lt;0.001), diene conjugates by 2.4 times (p&lt;0.001) and an increase in the activity of catalase by 1.5 times (p&lt;0.01), and superoxide dismutase by 1.6 times (p&lt;0.001) was found. The use of sea buckthorn oil led to a decrease in the number of Enterobacter by 1.6 times (p&lt;0.001), Morganella by 2.3 times (p&lt;0.001), and Acinetobacter by 2 times (p&lt;0.001). An increase in the activity of catalase by 1.4 times (p&lt;0.05), superoxide dismutase by 1.6 times (p&lt;0.001) and a decrease in the concentration of malondialdehyde by 2.9 times (p&lt;0.001), diene conjugates by 1.9 times (p&lt;0.001). Conclusion:&nbsp;The use of E and sea buckthorn oil increase protective properties of body during experimental dysbiosis.

Background:&nbsp;When the macroorganism is exposed to pesticides, qualitative and/or quantitative changes in the microbiocenosis composition occur &ndash; dysbiosis. As a result of this action, lipid peroxidation is enhanced and work of the body&#39;s antioxidant system is disrupted. The aim of the study:&nbsp;To study the state of colon microbiota and antioxidant properties of colonocytes in rats with subchronic thiram intoxication treated with vitamin E and sea buckthorn oil. Materials and methods:&nbsp;Animals in the experiment received thiram. Vitamin E and sea buckthorn oil were used as antioxidants. The composition of rat colon mucosal microflora was judged. The activity of antioxidant protection enzymes of colonocytes was studied by content of superoxide dismutase and catalase. The state of lipid peroxidation was assessed by content of diene conjugates and malondialdehyde. Results:&nbsp;The administration of thiram led to a decrease in the number of Bifidobacteria by 3 times (p&lt;0.001), Lactobacillus by 2.5 times (p&lt;0.001), E. coli with normal enzymatic activity by 2.2 times (p&lt;0.001), Escherichia with reduced enzymatic activity by 2.8 times (p&lt;0.001), Enterobacter by 1.8 times (p&lt;0.001), Salmonella by 1.7 times (p&lt;0.01), Citrobacter by 1.75 times (p&lt;0.01), coagulase-negative Staphylococci by 2.1 times (p&lt;0.001), Enterococci by 1.5 times (p&lt;0.05). There was a decrease in the activity of catalase by 1.9 times (p&lt;0.001), superoxide dismutase by 2.3 times (p&lt;0.001) and an increase in the concentration of malondialdehyde by 2.6 times (p&lt;0.001), diene conjugates by 2.4 times (p&lt;0.001). The use of vitamin E contributed to an increase in the number of Lactobacillus by 1.4 times (p&lt;0.05), a decrease in the number of Enterobacter, Morganella and Acinetobacter by 1.5 times (p&lt;0.05), 2 times (p&lt;0.001) and 1.8 times (p&lt;0.01), respectively. A decrease in the content of malondialdehyde by 3 times (p&lt;0.001), diene conjugates by 2.4 times (p&lt;0.001) and an increase in the activity of catalase by 1.5 times (p&lt;0.01), and superoxide dismutase by 1.6 times (p&lt;0.001) was found. The use of sea buckthorn oil led to a decrease in the number of Enterobacter by 1.6 times (p&lt;0.001), Morganella by 2.3 times (p&lt;0.001), and Acinetobacter by 2 times (p&lt;0.001). An increase in the activity of catalase by 1.4 times (p&lt;0.05), superoxide dismutase by 1.6 times (p&lt;0.001) and a decrease in the concentration of malondialdehyde by 2.9 times (p&lt;0.001), diene conjugates by 1.9 times (p&lt;0.001). Conclusion:&nbsp;The use of E and sea buckthorn oil increase protective properties of body during experimental dysbiosis.

colon microbiocenosisecological dysbiosisthiramantioxidant systemlipid peroxidationvitamin Esea buckthorn oilcolon microbiocenosisecological dysbiosisthiramantioxidant systemlipid peroxidationvitamin Esea buckthorn oilСписок литературыGilbert JA, Blaser MJ, Caporaso JG, et. al. Current understanding of the human microbiome. Nature Medicine. 2018;24(4):392-400. DOI: https://doi.org/10.1038/nm.4517Seliverstov PV, Sitkin SI, Radchenko VG, et al. Saccharomyces boulardii modulates the composition of the gut microbiota in patients with non-alcoholic fatty liver disease, thus preventing the progression of the disease. Experimental and Clinical Gastroenterology. 2018;2:4-18. Russian.Opazo MC, Ortega-Rocha EM, Coronado-Arr&aacute;zola I, et. al. Intestinal microbiota influences non-intestinal related autoimmune diseases. Frontiers in Microbiology. 2018;9:432. DOI: https://doi.org/10.3389/fmicb.2018.00432Lazebnik LB, Konev YuV. Microbiota, dysbiosis and age-related diseases. Clinical Gerontology. 2020;26(1-2):43-50. Russian. DOI: https://doi.org/10.26347/1607-2499202001-02043-050Shokirov B, Khalimova Yu. Antibiotic-induced rat gut microbiota dysbiosis and salmonella resistance. Society and innovations. 2021;4:94-100. Russian.Krasnikova EN, Danilova LA. Superoxide dismutase activity in children with psoriasis. Medicine: Theory and Practice. 2019;4:282-283. Russian.Nikitina OA. Activity of Lipoperoxidation &ndash; Antioxidant Protection Reactions in Patients with HIV Infection (Review). Acta Biomedica Scientifica. 2020;5(6):124-132. Russian. DOI: https://doi.org/10.29413/ABS.2020-5.6.14Maksimova VP, Usalka OG, Patsyurkevich AA, et al. Influence of thiram fungicide on signaling pathways involved in carcinogenesis. Crimea Journal of Experimental and Clinical Medicine. 2020;10(3):100. Russian.Korolev VA, Medvedeva OA, Ryadnova VA et al. Analysis of Thiram derivatives use in plant complex of the Kursk region. RUDN Journal of Ecology and Life Safety. 2020;28(2):103-111. Russian. DOI: https://doi.org/10.22363/2313-2310-2020-28-2-103-111State catalogue of pesticides and agrochemicals permitted for use on the territory of the Russian Federation. Moscow; 2021. Russian.Shevkoplyas-Gurieva NA, Sivkova GA. The use of pesticides and their impact on the environment and human health. Innovatsionnaya nauka. 2020;12:15-16. Russian.Ivanova MK, Tretyakova YuD. Antioxidant drugs in the chronopharmacological approach to the treatment of withdrawal symptoms. International Journal of Humanities and Natural Sciences. 2021;4-2(55):143-146. Russian. DOI: https://doi.org/10.24412/2500-1000-2021-4-2-143-146Kuleshova ON, Teply DL, Teply DD. Correction of Free Radical Blood Imbalance and Behaviour in Prenatally Stressed Rats with &alpha;-Tocopherol. Journal of Medical and Biological Research. 2021;9(1):25-34. Russian. DOI: https://doi.org/10.37482/2687-1491-Z040Kukina TP, Shcherbakov DN, Gensh KV, et al. Bioactive components of ethereal extract of wood greening sea buckthorn Hippophae rhamnoides L. Khimiya Rastitel&#39;nogo Syr&#39;ya. 2019;1:157-164. Russian. DOI: https://doi.org/10.1425 8/j cprm.2019014340Belov DA. Chemical methods and means of plant protection in forestry and landscaping: a textbook for students. Moscow: MGUL; 2003. Russian.Khabriev RU. Guidelines for the experimental (preclinical) study of new pharmacological substances. Moscow: OAO &laquo;Meditsina&raquo;; 2005. Russian.Efimov BA, Kafarskaya LI, Korshunov VM. Modern methods for assessing the qualitative and quantitative indicators of intestinal and vaginal microflora. Zhurnal mikrobiologii, &egrave;pidemiologii i immunobiologii. 2002;4:72-78. Russian.Vorobyov AA, Nesvizhsky YuV., Bogdanova EA, et al. Study of the parietal microflora of rats intestines. Zhurnal mikrobiologii, &egrave;pidemiologii i immunobiologii. 2005;3:61-65. Russian.Vorobyov AA, Nesvizh YuV, Bogdanova EA, et al. Features of microbiocenosis of the parietal mucin of the rats gastrointestinal tract. Zhurnal mikrobiologii, &egrave;pidemiologii i immunobiologii. 2005;6:3-7. Russian.Nesvizhsky YuV, Bogdanova EA, Zverev VV, et al. Microbiocenosis of parietal murocm in gastrointestinal tract of rats with induced disbiosis. Zhurnal mikrobiologii, &egrave;pidemiologii i immunobiologii. 2007;3:57-60. Russian.Malikhova OA, Karasev IA, Davydkina TS, et al. Intestinal microbiome and colorectal cancer. Literature review. Oncology Bulletin of the Volga region. 2019;10(4):45-51. Russian.Bagirov NS, Petukhov IN, Dmitriev NV, et al. Microbiome and cancer: is there a connection? Literature review. Malignant Tumours. 2018;3s1:56-69. Russian. DOI: https://doi.org/10.18027/2224-5057-2018-8-3s1-56-69Kornienko EA. Intestinal microbiota as a key factor in the formation of immunity and tolerance. Probiotics capabilities. Meditsinskiy sovet = Medical Council. 2020;10:92-100. Russian. DOI: https://doi.org/10.21518/2079-701X-2020-10-92-100Gurova NE, Sumnaya DB, Sadova VA. Changes in the system lipid peroxidation &ndash; antioxidant system (POL-AOS) in treating coxarthrosis with hyaluronic acid preparations. Sciences of Europe. 2019;35:43-46. Russian.Toboev GV, Aliev KI. Effects of Melaxen on the indices of lipid peroxidation and antioxidant deffence in experiment in chronic odontogenic osteomyelitis of jaws. Siberian Journal of Life Sciences and Agriculture. 2019;11(4):118-126. Russian. DOI: https://doi.org/10.12731/2658-6649-2019-11-4-118-126Isomadinova GZ, Bektemirova ZO. Changes in the intensity of the antioxidant and oxidant systems in the organs of rats with liver pathology. Forcipe. 2021;4(S1):476. Russian.Sitkin SI, Vakhitov TYa, Demyanova EV. Microbiome, gut dysbiosis and inflammatory bowel disease: That moment when the function is more important than taxonomy. Almanac of Clinical Medicine. 2018;46(5):396-425. Russian. DOI: https://doi.org/10.18786/2072-05-05-2018-46-5-396-425Zabolotneva AA, Shatova OP, Mikin IE, et al. Regulatory role and anticarcinogenic properties of certain vitamins&#39; active derivatives and vitamin-like substances. Problems of Nutrition. 2022;91(1):53-64. Russian. DOI: https://doi.org/10.33029/0042-8833-2022-91-1-53-64Averyanova EV, Shkolnikova MN, Rozhnov ED, et al. Study of the biological activity of sea buckthorn meal flavonoids using specific biotest systems. Khimija rastitel&#39;nogo syr&#39;ja. 2020;4:235-241. Russian. DOI: https://doi.org/10.14258/jcpim.2020048859