2658-6533Research Results in Biomedicine2658-653310.18413/2658-6533-2023-9-2-0-23073Genetics<strong>Association between mitochondrial DNA copy number and high viral load in women with high-risk human papillomavirus</strong><strong>Association between mitochondrial DNA copy number and high viral load in women with high-risk human papillomavirus</strong>AlBosaleAbbas H.H.AlBosaleAbbas H.H.abbashammadi4@gmail.comMashkinaElena V.MashkinaElena V.lenmash@mail.ru20239200

Background:&nbsp;The infection that most often leads to cervical cancer is human papillomavirus (HPV) infection. A sign that the HPV infection might develop into cancer is mitochondrial dysfunction and DNA damage. Increased mitochondrial DNA copy number (mtCN) has been associated with cervical neoplasm as a compensatory mechanism for mitochondrial dysfunction. The aim of the study:&nbsp;To compare the variation of the mitochondrial DNA levels in the women&#39;s samples with high HPV load and the control group. Materials and methods:&nbsp;An investigation was carried out using a sample of 100 women. Half of the sample had been diagnosed with HPV. The HPV-DNA was analyzed using an Amplisens HPV HCR screen-titre-FRT PCR kit, while real-time PCR was employed to ascertain the relative mitochondrial DNA copy number content. Results:&nbsp;A statistical analysis found no correlation between mtCN and age for both sample cases and controls (r = - 0.11 and p = 0.44; r = 0.053, p = 0.71, respectively). A high level of infection was associated with a higher relative mitochondrial DNA content, in comparison with levels observed in the sample of healthy women (25.78 versus 18.13, respectively). Conclusion:&nbsp;This study significantly enhances the evidence that the mtCN is linked to an increased risk of high HPV load.

Background:&nbsp;The infection that most often leads to cervical cancer is human papillomavirus (HPV) infection. A sign that the HPV infection might develop into cancer is mitochondrial dysfunction and DNA damage. Increased mitochondrial DNA copy number (mtCN) has been associated with cervical neoplasm as a compensatory mechanism for mitochondrial dysfunction. The aim of the study:&nbsp;To compare the variation of the mitochondrial DNA levels in the women&#39;s samples with high HPV load and the control group. Materials and methods:&nbsp;An investigation was carried out using a sample of 100 women. Half of the sample had been diagnosed with HPV. The HPV-DNA was analyzed using an Amplisens HPV HCR screen-titre-FRT PCR kit, while real-time PCR was employed to ascertain the relative mitochondrial DNA copy number content. Results:&nbsp;A statistical analysis found no correlation between mtCN and age for both sample cases and controls (r = - 0.11 and p = 0.44; r = 0.053, p = 0.71, respectively). A high level of infection was associated with a higher relative mitochondrial DNA content, in comparison with levels observed in the sample of healthy women (25.78 versus 18.13, respectively). Conclusion:&nbsp;This study significantly enhances the evidence that the mtCN is linked to an increased risk of high HPV load.

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