2658-6533Research Results in Biomedicine2658-653310.18413/2658-6533-2024-10-1-0-103341Medicine (miscellaneous)<strong>The effect of interleukin-10 gene polymorphism in the formation of deep disorders of vaginal microbiocenosis in bacterial vaginosis</strong><strong>The effect of interleukin-10 gene polymorphism in the formation of deep disorders of vaginal microbiocenosis in bacterial vaginosis</strong>RakhmatullaevaMakhfuza M.RakhmatullaevaMakhfuza M.mahfuzar@inbox.ru202410100

Background: Bacterial vaginosis (BV) is a common vaginal infection that causes significant gynecological and obstetric morbidity. Despite modern methods and treatment regimens, BV therapy remains a difficult task due to high rates of relapses, which amount to 60% within a year after treatment. This problem, in part, can be solved by assessing the individual genetic characteristics of the organism associated with the presence of allelic variants of the immune system genes that determine the constitutional possibility of a high or low level of synthesis of the corresponding immunocompetent molecules (cytokines, pattern-recognition receptors, etc.). The aim of the study: To determine the significance of the polymorphism -1082 G/A (rs1800896) of the gene IL-10 in the formation of various molecular biological profiles of vaginal microbiocenosis in bacterial vaginosis. Materials and methods: 195 women of reproductive age were examined, including 100 patients with bacterial vaginosis and 95 conditionally healthy women with vaginal normocenosis. The state of vaginal microbiocenosis was assessed by microscopic and molecular biological methods. The polymorphism -1082 G/A (rs1800896) of the IL-10 gene was genotyped using polymerase chain reaction and detection of amplification products on an electrophoresis gel. Results: Genotype G/G was more common in women with the prevalence of obligate-anaerobic microorganisms in the microbiocenosis (21.2%, p=0.046), genotype G/A &ndash; in women with severe mixed dysbiosis (61.3%, p=0.023). In women with a predominance of facultative anaerobes Gardnerella vaginalis and Atopobium vaginae in the microbiocenosis, there was no significant difference in the distribution of genotypes (p&gt;0.05). Conclusion: The carriage of allelic variants -1082G (rs1800896) of the gene IL-10 is associated with deeper disorders of vaginal microbiocenosis, which suggests their use as markers that increase the effectiveness of the diagnosis of recurrent forms of bacterial vaginosis

Background: Bacterial vaginosis (BV) is a common vaginal infection that causes significant gynecological and obstetric morbidity. Despite modern methods and treatment regimens, BV therapy remains a difficult task due to high rates of relapses, which amount to 60% within a year after treatment. This problem, in part, can be solved by assessing the individual genetic characteristics of the organism associated with the presence of allelic variants of the immune system genes that determine the constitutional possibility of a high or low level of synthesis of the corresponding immunocompetent molecules (cytokines, pattern-recognition receptors, etc.). The aim of the study: To determine the significance of the polymorphism -1082 G/A (rs1800896) of the gene IL-10 in the formation of various molecular biological profiles of vaginal microbiocenosis in bacterial vaginosis. Materials and methods: 195 women of reproductive age were examined, including 100 patients with bacterial vaginosis and 95 conditionally healthy women with vaginal normocenosis. The state of vaginal microbiocenosis was assessed by microscopic and molecular biological methods. The polymorphism -1082 G/A (rs1800896) of the IL-10 gene was genotyped using polymerase chain reaction and detection of amplification products on an electrophoresis gel. Results: Genotype G/G was more common in women with the prevalence of obligate-anaerobic microorganisms in the microbiocenosis (21.2%, p=0.046), genotype G/A &ndash; in women with severe mixed dysbiosis (61.3%, p=0.023). In women with a predominance of facultative anaerobes Gardnerella vaginalis and Atopobium vaginae in the microbiocenosis, there was no significant difference in the distribution of genotypes (p&gt;0.05). Conclusion: The carriage of allelic variants -1082G (rs1800896) of the gene IL-10 is associated with deeper disorders of vaginal microbiocenosis, which suggests their use as markers that increase the effectiveness of the diagnosis of recurrent forms of bacterial vaginosis

bacterial vaginosispolymorphism -1082 G/A (rs1800896) of the gene IL-10vaginal microbiocenosisanti-inflammatory cytokinesbacterial vaginosispolymorphism -1082 G/A (rs1800896) of the gene IL-10vaginal microbiocenosisanti-inflammatory cytokinesСписок литературыPeebles K, Velloza J, Balkus JE, et al. High global burden and costs of bacterial vaginosis: A systematic review and meta-analysis. Sexually Transmitted Diseases. 2019;46(5):304-311. DOI: https://doi.org/10.1097/OLQ.0000000000000972Barinov SV, Okhlopkov VA, Tirskaya YuI, et al. Genital microbiota of sexual partners of female patients with bacterial vaginosis is responsible for its recurrences. Fundamental and Clinical Medicine. 2020;5(3):66-76. Russian. DOI: https://doi.org/10.23946/2500-0764-2020-5-3-66-76Sanchez-Garcia EK, Contreras-Paredes A, Martinez-Abundis E, et al. Molecular epidemiology of bacterial vaginosis and its association with genital micro-organisms in asymptomatic women. Journal of Medical Microbiology. 2019;68(9):1373-1382. DOI: https://doi.org/10.1099/jmm.0.001044Muzny CA, Łaniewski P, Schwebke JR, et al. Host-vaginal microbiota interactions in the pathogenesis of bacterial vaginosis. Current Opinion in Infectious Disease. 2020;33(1):59-65. DOI: https://doi.org/10.1097/QCO.0000000000000620Maryanyan AYu, Slepchenko VV, Rashidova MA, et al. Current ideas about the specific features of vaginal microbiocenosis in HIV-positive women of reproductive age. Obstetrics and gynecology. 2019;12:12-17. Russian. DOI: https://dx.doi.org/10.18565/aig.2019.12.12-17Chao X-P, Sun T-T, Wang S, et al. Correlation between the diversity of vaginal microbiota and the risk of high-risk human papillomavirus infection. International Journal of Gynecological Cancer. 2019;29:28-34. DOI: https://doi.org/10.1136/ijgc-2018-000032Gondwe T, Ness R, Totten PA, et al. Novel bacterial vaginosis-associated organisms mediate the relationship between vaginal douching and pelvic inflammatory disease. Sexually Transmitted Infections. 2020;96(6):439-444. DOI: https://doi.org/10.1136/sextrans-2019-054191Sinyakova AA, Shipitsyna EV, Budilovskaya OV, et al. Clinical, anamnestic and microbiological predictors of miscarriage. Journal of Obstetrics and Women&#39;s Diseases. 2019;68(2):59-70. Russian. DOI: https://doi.org/10.17816/JOWD68259-70Fettweis JM, Serrano MG, Brooks JP, et al. The vaginal microbiome and preterm birth. Nature Medicine. 2019;25(6):1012-1021. DOI: https://doi.org/10.1038/s41591-019-0450-2Provotorova TV. Analysis of long-term results of Metrogil vaginal gel and Metrogil plus in the treatment of recurrent vaginal biocenosis disorders. Obstetrics, Gynecology and Reproduction. 2018;12(2):32-38. Russian. DOI: https://doi.org/10.17749/2313-7347.2018.12.2.032-038Savicheva AM, Tapilskaya NI, Krysanova AA, et al. Long-term results of two-stage treatment of bacterial vaginosis using antiseptics and probiotics. Obstetrics and Gynecology: News, Opinions, Training. 2021;9(4):19-28. Russian. DOI: https://doi.org/10.33029/2303-9698-2021-9-4-19-28Laniewski P, Herbst-Kralovetz MM. Bacterial vaginosis and health-associated bacteria modulate the immunometabolic landscape in 3D model of human cervix.&nbsp;npj Biofilms and Microbiomes. 2021;7:88. DOI: https://doi.org/10.1038/s41522-021-00259-8Campisciano G, Zanotta N, Licastro D, et al. In vivo microbiome and associated immune markers: new insights into the pathogenesis of vaginal dysbiosis. Scientific Reports. 2018;8:2307. DOI: https://doi.org/10.1038/s41598-018-20649-xSaraiva M, Vieira P, O&#39;Garra A. Biology and therapeutic potential of interleukin-10. Journal of Experimental Medicine. 2020;217(1):e20190418. DOI: https://doi.org/10.1084/jem.20190418Islam H, Chamberlain TC, Mui AL, et al. Elevated Interleukin-10 Levels in COVID-19: Potentiation of Pro-Inflammatory Responses or Impaired Anti-Inflammatory Action? Frontiers in Immunology. 2021;12:677008. DOI: https://doi.org/10.3389/fimmu.2021.677008De Seta F, Campisciano G, Zanotta N, et al. The vaginal community state types microbiome-immune network as key factor for bacterial vaginosis and aerobic vaginitis. Frontiers in Microbiology. 2019;10:2451. DOI: https://doi.org/10.3389/fmicb.2019.02451Tarique M, Naz H, Saini C, et al. Association of IL-10 Gene Polymorphism With IL-10 Secretion by CD4 and T Regulatory Cells in Human Leprosy. Frontiers in Immunology. 2020;11:1974. DOI: https://doi.org/10.3389/fimmu.2020.01974Mehta SD, Nannini DR, Otieno F, et al. Host genetic factors associated with vaginal microbiome composition in Kenyan women. mSystems. 2020;5(4):e00502-20. DOI: https://doi.org/10.1128/mSystems.00502-20Taylor BD, Totten PA, Astete SG, et al. Toll-like receptor variants and cervical Atopobiumvaginae infection in women with pelvic inflammatory disease. American Journal of Reproductive Immunology. 2018;79(2):e12804. DOI: https://doi.org/10.1111/aji.12804Nazarova VV, Shipitsyna EV, Gerasimova EN, et al. Criteria for the diagnosis of bacterial vaginosis using the Femoflor-16 test. Journal of Obstetrics and Women&#39;s Diseases. 2017;66(4):57-67. Russian. DOI: https://doi.org/10.17816/JOWD66457-67Vestby LK, Grоnseth T, Simm R, et al. Bacterial Biofilm and its Role in the Pathogenesis of Disease. Antibiotics. 2020;9(2):59. DOI: https://doi.org/10.3390/antibiotics9020059Goncharova IA, Bragina EYu, Zhalsanova IZ, et al. Effect of IL10 (rs1800872) and CXCL10 (rs4386624, rs4256246) genes polymorphism in the development of viral and bacterial infectious diseases. Research Results in Biomedicine. 2019;5(4):32-43. Russian. DOI: https://doi.org/10.18413/2658-6533-2019-5-4-0-3Jafarova TS. Single nucleotide polymorphism at position -1082 (a/g) in interleukin-10 gene promoter region in the newborns with necrotic enterocolitis. Journal of New Medical Technologies. 2020;27(3):38-41. Russian. DOI: https://doi.org/10.24411/1609-2163-2020-16645Khorrami S, Zamani H, Hasanzadeh M, et al. Association of a genetic variant in Interleukin-10 gene with increased risk and inflammation associated with cervical cancer. Gene. 2022;807:145933. DOI: https://doi.org/10.1016/j.gene.2021.145933Pavlova AA, Dolgushina NV, Donnikov AE, et al. Estimation of cytokine gene expression and polymorphism as predictors for chronic allergic vulvovaginitis. Obstetrics and Gynecology. 2016;3:64-70. Russian. DOI: http://dx.doi.org/10.18565/aig.2016.3.64-70Kazakova AV, Uvarova EV, Bezrukova AA, et al. Prediotion of inflammatory diseases of the vulva and vagina in girls depending on the stage of sexual development. Aspirantskiy Vestnik Povolzhiya. 2019;19(1-2):47-53. Russian. DOI: https://doi.org/10.17816/2072-2354.2019.19.1.47-53