2658-6533Research Results in Biomedicine2658-653310.18413/2313-8955-2015-1-4-74-78501PharmacologyPHARMACOLOGICAL PRECONDITIONING WITH NICORANDIL AS A POSSIBILITY OF INCREASING THE STABILITY OF TISSUES OF THE RETINA TO ISCHEMIA IN AN EXPERIMENTPHARMACOLOGICAL PRECONDITIONING WITH NICORANDIL AS A POSSIBILITY OF INCREASING THE STABILITY OF TISSUES OF THE RETINA TO ISCHEMIA IN AN EXPERIMENTShabelnikovaAnna S.ShabelnikovaAnna S.shabelnikova@bsu.edu.ru20151400

In laboratory rats, we formed an ischemic damage by providing mechanical pressure (110 mm Hg) to the anterior chamber for 30 minutes. The experiment has revealed the fact that nicorandil (0.6 mg/kg) prevents the development of degenerative changes in the retinal layers induced by ischemic injury. The severity of the protective effect of nicorandil on the retina of the rats was assessed after 72 hours of reperfusion after modeling the pathology. We have found a protective effect of nicorandil with the development of ischemia, which was confirmed by the laser Doppler flowmetry and histomorphometry. The identification and application of pharmacological agents with the effect of preconditioning may be a novel approach in the correction and prevention of ischemia of the retina which is the leading element in the pathogenesis of a number of eye pathologies. The possibility of pharmacological preconditioning with nicorandil of ischemic damage to the retina is essential for the development of anti-ischemic agents for the treatment and prevention of ophthalmic pathologies of an ischemic genesis.

In laboratory rats, we formed an ischemic damage by providing mechanical pressure (110 mm Hg) to the anterior chamber for 30 minutes. The experiment has revealed the fact that nicorandil (0.6 mg/kg) prevents the development of degenerative changes in the retinal layers induced by ischemic injury. The severity of the protective effect of nicorandil on the retina of the rats was assessed after 72 hours of reperfusion after modeling the pathology. We have found a protective effect of nicorandil with the development of ischemia, which was confirmed by the laser Doppler flowmetry and histomorphometry. The identification and application of pharmacological agents with the effect of preconditioning may be a novel approach in the correction and prevention of ischemia of the retina which is the leading element in the pathogenesis of a number of eye pathologies. The possibility of pharmacological preconditioning with nicorandil of ischemic damage to the retina is essential for the development of anti-ischemic agents for the treatment and prevention of ophthalmic pathologies of an ischemic genesis.

ischemia-reperfusion of the retinapharmacological preconditioningnicorandilATP-sensitive potassium channelsischemia-reperfusion of the retinapharmacological preconditioningnicorandilATP-sensitive potassium channelsСписок литературыKolesnik I.M., Efremenkova D.A., Pokrovsky M.V. / Pharmacological Preconditioning with Nicorandil and Minoxidil - the Possibility of Use in Surgery // Herald of VolGMU: Annex (Proceedings of the IV All-Russian scientific-practical seminar of young scientists with international participation «Modern Problems of Medical Chemistry. Directed Search for New Medicines»). 2012. P.148.Peresypkina, A.A., Pokrovskiy M.V., Shabelnikova A.S., Kaszuba A.S., Dolzhikov A.A. and  Pirogov A.V.. Electrophysiological and Histomorphometric Changes in the Rat Retina in Pharmacological Preconditioning with Erythropoietin Nicorandil on the model of ischemia-reperfusion of the eye. The Scientific Journal of Belgorod State University. Series: Medicine. Pharmacy, 11 (182): 113-117.Shabelnikova A.S., Kashuba A.S., Peresypkina А.А., Pokrovskiy M.V, Dolzhikov  A.A., Buslovskaya L.K. Protective Effect of Erythropoietin in the Simulation of Retinal Ischemia-reperfusion. The Scientific Journal Belgorod State University. Series: Medicine. Pharmacy, 2014.  11 (182): 109-112.Adnan I. Qureshi. Textbook of Interventional Neurology. Cambridge: Cambridge University Press, 2011. P.506.Cardioprotective effects of sarcolemmal and mitochondrial K-ATP channel openers in an experimental model of autoimmune myocarditis. Role of the reduction in calcium overload during acute heart failure / Niwano S, Hirasawa S, Niwano H, Sasaki S, Masuda R, Sato K, Masuda T, Izumi T // Int Heart J. 53, 2 (2012): 139-145.Cervia D, Casini G. The Neuropeptide Systems and their Potential Role in the Treatment of Mammalian Retinal Ischemia: A Developing Story//Curr Neuropharmacol. 11, 1 (2013): 95–101.Gillipsie Minhas, Akshay Anand. Animal Models of Retinal Ischemia. Rijeka: InTech, 2011. P. 522.Massive subretinal bleed in a patient with background diabetic retinopathy and on treatment with warfarin / Raj A, Sekhri R, A Salam and P Priya // Eye. 17, (2003): 649–652.Mitochondrial K(ATP) channel opener prevents ischemia-reperfusion injury in rat liver / Hai S, Takemura S, Minamiyama Y, Yamasaki K, Yamamoto S, Kodai S, Tanaka S, Hirohashi K, Suehiro S // Transplant Proc. 37, 1 (2005): 428-31.Pharmacological preconditioning of ATP-sensitive potassium channel openers on acute urinary retention-induced bladder dysfunction in the rat / Ohmasa F, Saito M, Oiwa H, Tsounapi P, Shomori K, Kitatani K, Dimitriadis F, Kinoshita Y, Satoh K. // BJU Int. 110, 6 Pt B (2012):E245-52.