2658-6533Research Results in Biomedicine2658-653310.18413/2313-8955-2015-1-4-119-124509Archive categoriesTHE CHEMICAL STUDY OF THE ANTHOCYANINS IN <em>OCIMUM BASILICUM</em> L. HERBTHE CHEMICAL STUDY OF THE ANTHOCYANINS IN <em>OCIMUM BASILICUM</em> L. HERBSevrukInna A.SevrukInna A.sevruk@bsu.edu.ruNovikovOleg O.NovikovOleg O.ole9222@yandex.ruPisarevDmitri I.PisarevDmitri I.juniper05@mail.ruKornienkoIrina V.KornienkoIrina V.indina@bsu.edu.ruAlekseevaKseniya A.AlekseevaKseniya A.740890@bsu.edu.ru20151400

Sweet basil &minus; Ocimum basilicum L. contains a wide spectrum of biologically active compounds: essential oils, phenolic compounds, including flavonoids and anthocyanins. The violet variety is a rich source of acylated and glycosylated anthocyanins and can be of interest as a unique source of stable red pigments for the food industry. The HPLC method has determined the chemical composition of O. basilicum L. anthocyanins: it contains 12 anthocyanin glycosides, and these compounds are in the form of depsides with hydroxy-cinnamic acids, as evidenced by their characteristic UV profiles. The aglycone of anthocyanin glycosides is cyanidin, which was confirmed by the acid hydrolysis. And 11 anthocyanin glycosides are represented as depsides with p-coumaric acid. One anthocyan glycoside is in the form of a depside with caffeic acid. The quantification of anthocyanins was determined by the pH-differential spectrophotometry. It was found that the fresh O. basilicum L. raw material total content of anthocyanins was &ndash; 0,48 &plusmn; 0,054%.

Sweet basil &minus; Ocimum basilicum L. contains a wide spectrum of biologically active compounds: essential oils, phenolic compounds, including flavonoids and anthocyanins. The violet variety is a rich source of acylated and glycosylated anthocyanins and can be of interest as a unique source of stable red pigments for the food industry. The HPLC method has determined the chemical composition of O. basilicum L. anthocyanins: it contains 12 anthocyanin glycosides, and these compounds are in the form of depsides with hydroxy-cinnamic acids, as evidenced by their characteristic UV profiles. The aglycone of anthocyanin glycosides is cyanidin, which was confirmed by the acid hydrolysis. And 11 anthocyanin glycosides are represented as depsides with p-coumaric acid. One anthocyan glycoside is in the form of a depside with caffeic acid. The quantification of anthocyanins was determined by the pH-differential spectrophotometry. It was found that the fresh O. basilicum L. raw material total content of anthocyanins was &ndash; 0,48 &plusmn; 0,054%.

basil ordinarydepsides anthocyanin glycosideshigh performance liquid chromatographybasil ordinarydepsides anthocyanin glycosideshigh performance liquid chromatographyСписок литературыPlant Biochemistry / L.A Krasil&#39;nikova, O.A Avksentiev Yu.А. Zhmurko [et al.]. Rostov n/D: Phoenix; Kharkov: Torsing, 2004. 224 p.Geyscman, T. Anthocyanins, chalcones, auron, flavones and related water-soluble pigments // Biochemical methods of analysis plan. M., 1960. P. 453.Kishkovsky, Z. H., Skurihin, I.M. Chemistry wine. M.: Food Industry, 1976. 312 p.Methods of analysis of biologically active substances minor food / ed. VA Tutelian, K.I. Eller; Institute of Nutrition. M.: Dynasty, 2010. 180 p.Tanchev, S.S. Anthocyanins in fruits and vegetables. M.: Food Industry, 1980. 302 p.Analysis and biological activities of anthocyanin / J. M. Kong, L. S. Chia, N. K. Goh [et al.] // Phytochemistry. Vol.64, №5. 2003: P. 923-933.Antal, D.-S, G&acirc;rban, G., G&acirc;rban, Z. The anthocyans: biologically-active substances of food and pharmaceutic interest // The Annals of the University Dunarea de Jos of Galati. Fascicle VI : Food Technology. 2003: P. 106-115.Antioxidant and antiinflammatory activities of anthocyanins and their aglycon, cyanidin, from tart cherries / H. Wang, M. G. Nair, G. M. Strasburg [et al.] // J. Nat. Prod. Vol.62, №2. 1999: P. 294-296.Phippen, B.W., Simon, J.E. Anthocyanins in Basil (Ocimum basilicum&nbsp;L.) // J. Agric. Food Chem. Vol.46, №5. 1998: P. 1734&ndash;1738.Expression of genes involved in anthocyanin biosynthesis in relation to anthocyanin, proanthocyanidin, and flavonol levels during bilberry fruit development / L. Jaakola, K. M&auml;&auml;tt&auml;, A. M. Pirttil&auml; [et al.] // Plant. Physiol. Vol.130, №2. 2002: P. 729-739.Hou, D. X. Potential mechanisms of cancer chemoprevention by anthocyanins // Curr. Mol. Med. Vol.3, №2. 2003: P. 149-159.Lila, M. A. Anthocyanins and human health: an in vitro investigative approach // J. Biomed. Biotechnol. Vol.2004, №5. 2004: P. 306-313.Norton, R. A. Inhibition of Aflatoxin B1 Biosynthesis in Aspergillus flavus by Anthocyanidins and Related Flavonoids // J. Agric. Food. Chem. Vol.47, №3. 1999: P. 1230-1235.