Having achieved a great success in the field of synthetic drugs, currently much attention is paid to the development and introduction of effective herbal medicines. Great interest in wild plants of folk medicine is largely due to the broad possibilities of their use and also to extensive areas of their growth that allow to serve the needs of health care in medicinal plant raw materials. Among the rich flora of Russia, the representatives of the Fabaceae family, and Trifolium alpestre L. in particular, are of considerable interest. This article presents the results of the phytochemical study of the Trifolium alpestre L. herb. There was conducted a qualitative detection and quantitative determination of carbohydrates, tannins, organic acids, phenol carbonic acids and flavonoids in the object under the study. According to the results, the main active ingredient groups in the Trifolium alpestre L. herb are organic acids and phenolic compounds.