2658-6533Research Results in Biomedicine2658-653310.18413/2313-8955-2017-3-1-56-62999Archive categoriesTHE STUDY OF LIPOPHILIC SUBSTANCES OF TARAXACUM OFFICINALE WIGG HERBTHE STUDY OF LIPOPHILIC SUBSTANCES OF TARAXACUM OFFICINALE WIGG HERBLozovitskyDmitry A.LozovitskyDmitry A.20173100

In our paper, we provide some results of the research of photosynthetic pigments of Taraxacum officinale herb which grows in Altai region. The yield of lipophilic fraction was determined in different vegetation phases of the studied raw-material. By the TLC and spectrophotometry the following compounds were identified: β-carotene, lutein, violaxanthin, neoxanthin, taraxanthin, chlorophyll a and chlorophyll b. For the TLC analysis, the mobile phase of acetone-petroleum ether (3:7) proved to be the most efficient. The TLC method proposed in the paper can be used for inclusion dandelion (Taraxacum officinale) into the modern normative documents on medicinal raw material. The conditions of spectrophotometric method of pigments determination in Taraxacum officinale herb were experimentally chosen. The total amount of carotenoids and total amount of chlorophyll were determined for different vegetation phases of Taraxacum officinale herb. For using as a carotenoids source the most optimal vegetation phase for collection of raw material is the budding phase. The studied medicinal plant may find future application in clinical treatment of various diseases.

In our paper, we provide some results of the research of photosynthetic pigments of Taraxacum officinale herb which grows in Altai region. The yield of lipophilic fraction was determined in different vegetation phases of the studied raw-material. By the TLC and spectrophotometry the following compounds were identified: β-carotene, lutein, violaxanthin, neoxanthin, taraxanthin, chlorophyll a and chlorophyll b. For the TLC analysis, the mobile phase of acetone-petroleum ether (3:7) proved to be the most efficient. The TLC method proposed in the paper can be used for inclusion dandelion (Taraxacum officinale) into the modern normative documents on medicinal raw material. The conditions of spectrophotometric method of pigments determination in Taraxacum officinale herb were experimentally chosen. The total amount of carotenoids and total amount of chlorophyll were determined for different vegetation phases of Taraxacum officinale herb. For using as a carotenoids source the most optimal vegetation phase for collection of raw material is the budding phase. The studied medicinal plant may find future application in clinical treatment of various diseases.

dandelionTaraxacum officinaleherbcarotenoidscarotenexanthophyllchlorophylllipophilic substancesthin layer chromatographyspectrophotometrydandelionTaraxacum officinaleherbcarotenoidscarotenexanthophyllchlorophylllipophilic substancesthin layer chromatographyspectrophotometryСписок литературы Vetrov P.P., Garnaya S.V., Dolgonenko L.G. Determination of total amount of carotenoinds and lipophilic substances content in herbal raw-material. Pharmaceutical Chemistry Journal.  1989. 3. Pp. 320-325.The state pharmacopoeia of the Russian Federation. XIII edition. 2015. M., volume 1.Kuregyan A.G., Pechinskiy S.V., Zilfikarov I.N. Methods of obtaining carotenoids, medicines and food additives on the basis of their drug development and registration. 2014. URL: http://pharmjournal.ru/articles/stati/sposobyi-polucheniya-karotinoidov-lekarstvennyix-preparatov-i-biologicheski-aktivnyix-dobavok-k-pishhe-na-ix-osnove-6-fevral-2014 (date of access: January 20, 2017).Trineeva O.V., Slivkin A.I., Safonova E.F. Determination of hydroxycinnamic acids, carotenoids and chlorophyll in the leaves of nettle (Urtica Dioica L.). Chemistry of plant raw material. 2015. 3. Pp. 105-110. (in Russian)Trineeva O.V., Voropaeva S.V., Slivkin A.I. Selection of the optimum system for the determination of pigments of leaves of Urtica Dioica by the TLC method. Sorption and chromatographic processes. 2013. 13(2). Pp. 213-219.Fedoseeva L.M., Maloletkina T.S. The research and comparative evaluation of lipophilic substances of green, red and black leaves of bergenia crassifolia which grows in Altai. Chemistry of plant raw material. 1999. 2. Pp. 113-117.Checheta O.V., Safonova E.F., Slivkin A.I. Technique of carotenoids determination by the method of thin-layer chromatography. Sorption and chromatographic processes. 2008. 8 (2). Pp. 320-326.