2658-6533Научные результаты биомедицинских исследований2658-653310.18413/2658-6533-2026-12-2-0-74157Фармакология, клиническая фармакология<strong>Phytochemical and pharmacological properties of</strong><strong> acetone</strong><strong> extract from</strong> <strong><em>Curcuma vitellina</em></strong><br /> &nbsp;<strong>Phytochemical and pharmacological properties of</strong><strong> acetone</strong><strong> extract from</strong> <strong><em>Curcuma vitellina</em></strong><br /> &nbsp;PhamViet T.PhamViet T.phamtanviet@iuh.edu.vnVanThien H.VanThien H.vanhongthien@iuh.edu.vnDoLoi T.DoLoi T.thiloido2611@gmail.comNguyenVen T.NguyenVen T.nguyenthiven1902@gmail.comQuocLe P.T.QuocLe P.T.lephamtanquoc@iuh.edu.vnNguyenHung Q.NguyenHung Q.hungnq@case.vnLeTho T.LeTho T.tholt@case.vnDinhDiep Q.DinhDiep Q.dqdiep@gmail.comNguyen-PhiNgaNguyen-PhiNganpnga@hcmus.edu.vn202612200

Background: Curcuma vitellina has been recently described as a new species in the flora of Vietnam. To date, it is a rare species and its phytochemical and biological properties are limited. The aim of the study: The present study reports for the first time the chemical components and pharmacological properties of acetone extract of Curcuma vitellina, a native species from Vietnam. Materials and methods: The chemical components, antibacterial and antioxidant activities of the studied extract were identified using gas chromatography/mass spectrometry (GC/MS), agar disk-diffusion, and DPPH radical scavenging assays, respectively. Results: Forty-eight compounds were identified in the studied extract of which the major compounds were caryophyllene oxide (14.08%), (E)-labda-8(17),12-dien-15,16-dial (11.30%), longiverbenone (10.53%), 2-pentanone, 4-hydroxy-4-methyl- (9.98%), n-hexadecanoic acid (5.57%), and &gamma;-bicyclohomofarnesal (5.13%). In addition, the studied extract was found to be effective against Gram-positive strains, including S. aureus ATCC 29213, S. aureus ATCC 25923, B. cereus and S. saprophyticus whereas while four Gram-negative strains were not sensitive to this extract. The C. vitellina extract also presented the DPPH scavenging capacity with IC50&shy; value of 614.55 &micro;g/mL. Conclusion: The results from this report are to provide the potential application of the Curcuma vitellina extract in biomedicine and other related fields

Background: Curcuma vitellina has been recently described as a new species in the flora of Vietnam. To date, it is a rare species and its phytochemical and biological properties are limited. The aim of the study: The present study reports for the first time the chemical components and pharmacological properties of acetone extract of Curcuma vitellina, a native species from Vietnam. Materials and methods: The chemical components, antibacterial and antioxidant activities of the studied extract were identified using gas chromatography/mass spectrometry (GC/MS), agar disk-diffusion, and DPPH radical scavenging assays, respectively. Results: Forty-eight compounds were identified in the studied extract of which the major compounds were caryophyllene oxide (14.08%), (E)-labda-8(17),12-dien-15,16-dial (11.30%), longiverbenone (10.53%), 2-pentanone, 4-hydroxy-4-methyl- (9.98%), n-hexadecanoic acid (5.57%), and &gamma;-bicyclohomofarnesal (5.13%). In addition, the studied extract was found to be effective against Gram-positive strains, including S. aureus ATCC 29213, S. aureus ATCC 25923, B. cereus and S. saprophyticus whereas while four Gram-negative strains were not sensitive to this extract. The C. vitellina extract also presented the DPPH scavenging capacity with IC50&shy; value of 614.55 &micro;g/mL. Conclusion: The results from this report are to provide the potential application of the Curcuma vitellina extract in biomedicine and other related fields

acetone extractantibacterialantioxidant activitiesCurcuma vitellinaGC-MSacetone extractantibacterialantioxidant activitiesCurcuma vitellinaGC-MSСписок литературыLeong-&Scaron;korničkov&aacute; J, L&yacute; NS, Nguyễn QB. Curcuma arida and C. sahuynhensis, two new species from subgenus Ecomata (Zingiberaceae) from Vietnam. Phytotaxa. 2015;192(3):181-189. DOI: https://doi.org/10.11646/phytotaxa.192.3.4Rahaman MM, Rakib A, Mitra S, et al. The genus Curcuma and inflammation: Overview of the pharmacological perspectives. Plants. 2021;10(1):63. DOI: https://doi.org/10.3390/plants10010063Pham HH. Zingiberaceae. In: Pham-hoang H, editor. C&acirc;y cỏ Việt Nam: An Illustrated Flora of Vietnam, vol. 3. Ho Chi Minh City: Youth Publishing House; 2000.Nguyen QB. Zingiberaceae-Flora of Vietnam. Publishing House for Science and Technology; 2017.Akarchariya N, Sirilun S, Julsrigival J, et al. Chemical profiling and antimicrobial activity of essential oil from Curcuma aeruginosa Roxb., Curcuma glans K. Larsen &amp; J. Mood and Curcuma cf. xanthorrhiza Roxb. collected in Thailand. Asian Pacific Journal of Tropical Biomedicine. 2017;7(10):881-885. DOI: https://doi.org/10.1016/j.apjtb.2017.09.009.Rukayadi Y, Hwang J. In Vitro activity of xanthorrhizol isolated from the rhizome of Javanese turmeric (Curcuma xanthorrhiza Roxb.) against Candida albicans biofilms. Phytotherapy Research. 2013;27(7):1061-1066. DOI: https://doi.org/10.1002/ptr.4834Udin Z. Sitotoksisitas xanthorrhizol dari minyak atsiri rimpang Curcuma xanthorrhiza Roxb. terhadap sel kanker payudara YBM-1. Jurnal Kimia Terapan Indonesia. 2013;15(1):23-19. DOI: https://doi.org/10.14203/jkti.v15i1.101Forrer M, Kulik EM, Filippi A, et al. The antimicrobial activity of alpha-bisabolol and tea tree oil against Solobacterium moorei, a Gram-positive bacterium associated with halitosis. Archives of Oral Biology. 2013;58(1):10-16. DOI: https://doi.org/10.1016/j.archoralbio.2012.08.001Van HT, Phan UTX, Doan VD, et al. Chemical constituents of essential oil from rhizomes and aerial parts of Curcuma gracillima. Chemistry of Natural Compounds. 2021;57(3):569-571. DOI: https://doi.org/10.1007/s10600-021-03419-6Leong-&Scaron;korničkov&aacute; J, Tran HD, Newman M. Curcuma vitellina (Zingiberaceae), a new species from Vietnam. Gardens&rsquo; Bulletin Singapore. 2010;62(1):111-117.Nguyen-Phi N, Dinh DQ, Dinh ND, et al. Chemical components and antimicrobial activity of essential oil from Curcuma vitellina. Chemistry of Natural Compounds. 2024;60:356-358 DOI: https://doi.org/10.1007/s10600-024-04324-4Huynh TH, Tran GH, Trinh NN, et al. Chemical components and antioxidant properties of acetone extracts of four different species of Zingiberaceae. Plant Science Today. 2024;11(2):326-332. DOI: https://doi.org/10.14719/pst.3272Jorgensen JH, Hindler JA, Bernard K, et al. Methods for antimicrobial dilution and disk susceptibility of infrequently isolated or fastidious bacteria, approved guideline, 2nd. ed. CLSI document M45-A2. Wayne, PA: Clinical and Laboratory Standards Institute; 2010.Van HT, Tran MD, Tran TKN, et al. Chemical profiles and antibacterial activity of acetone extract of two Curcuma species from Vietnam. Plant Science Today. 2023;10(2):83-89. DOI: https://doi.org/10.14719/pst.1993Van HT, Luu TN, Pham TV, et al. Chemical constituents and antibacterial activity of essential oils from Curcuma thorelii. Chemistry of Natural Compounds. 2023;59:176-179. DOI: https://doi.org/10.1007/s10600-023-03947-3Van HT, Tran DT, Nguyen TH, et al. Chemical profiles and biological activities of acetone extracts of Curcuma xanthella. Herba Polonica. 2022;68(4):30-38. DOI: https://doi.org/10.2478/hepo-2022-0023Van HT, Pham TV, Le VS, et al. Chemical composition and antibacterial activity of essential oils from the aerial part of Curcuma xanthella. Chemistry of Natural Compounds. 2023;59(5):963-965. DOI: https://doi.org/10.1007/s10600-023-04165-7Dinh QD, Nguyen NA, Pham TV, et al. Essential oil from the aerial parts of Curcuma pygmaea: components and antibacterial activity. Chemistry of Natural Compounds. 2023;59(5):966-967. DOI: https://doi.org/10.1007/s10600-023-04166-6Sirat HM, Masri D, Rahman AA. The distribution of labdane diterpenes in the Zingiberaceae of Malaysia. Phytochemistry. 1994;36(3):699-701. DOI: https://doi.org/10.1016/S0031-9422(00)89800-3Awin T, Mediani A, Maulidiani M, et al. Phytochemical and bioactivity alterations of Curcuma species harvested at different growth stages by NMR-based metabolomics. Journal of Food Composition and Analysis. 2019;77:66-76. DOI: https://doi.org/10.1016/j.jfca.2019.01.004Rawat A, Prakash O, Kumar R, et al. Bioactive Compounds and Biological Activities of Hedychium Species. In: Murthy HN, Paek KY, Park SY, editors. Bioactive Compounds in the Storage Organs of Plants. Reference Series in Phytochemistry. Springer; 2024. DOI: https://doi.org/10.1007/978-3-031-44746-4_29Hamdi OAA, Rahman SNSA, Awang K, et al. Cytotoxic constituents from the rhizomes of Curcuma zedoaria. Scientific World Journal. 2014;2014:321943. DOI: https://doi.org/10.1155/2014/321943Fidyt K, Fiedorowicz A, Strządała L, et al. &beta;-caryophyllene and &beta;-caryophyllene oxide&mdash;natural compounds of anticancer and analgesic properties. Cancer Medicine. 2016;5(10):3007-3017. DOI: https://doi.org/10.1002/cam4.816Gyrdymova YV, Rubtsova SA. Caryophyllene and caryophyllene oxide: a variety of chemical transformations and biological activities. Chemical Papers. 2022;76:1-39. DOI: https://doi.org/10.1007/s11696-021-01865-8Balamurugan R, Duraipandiyan V, Ignacimuthu S. Antidiabetic activity of &gamma;-sitosterol isolated from Lippia nodiflora L. in streptozotocin induced diabetic rats. European Journal of Pharmacology. 2011;667(1-3):410-418. DOI: https://doi.org/10.1016/j.ejphar.2011.05.025Sundarraj S, Thangam R, Sreevani V, et al. &gamma;-Sitosterol from Acacia nilotica L. induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells. Journal of Ethnopharmacology. 2012;141(3):803-809. DOI: https://doi.org/10.1016/j.jep.2012.03.014Cheng Q, Zhang Y, Lin Q, et al. Study on the antioxidant activity of &beta;-sitosterol and stigmasterol from Sacha Inchi oil and Prinsepia oil added to walnut oil. Food Science and Technology. 2022;42:e69522. DOI: https://doi.org/10.1590/FST.69522Aurang ZM. Isolation and biological activity of &beta;-sitosterol and stigmasterol from the roots of&nbsp;Indigofera heterantha. Pharmacy and Pharmacology International Journal. 2017;5(5):204-207. DOI: https://doi.org/10.15406/ppij.2017.05.00139Paniagua-P&eacute;rez R, Flores-Mondrag&oacute;n G, Reyes-Legorreta C, et al. Evaluation of the anti-inflammatory capacity of beta-sitosterol in rodent assays. African Journal of Traditional, Complementary and Alternative Medicines. 2016;14(1):123-130. DOI: https://doi.org/10.21010/ajtcam.v14i1.13Fraile L, Crisci E, C&oacute;rdoba L, et al. Immunomodulatory properties of beta-sitosterol in pig immune responses. International Immunopharmacology. 2012;13(3):316-321. DOI: https://doi.org/10.1016/j.intimp.2012.04.017L&oacute;pez-Rubalcava C, Pi&ntilde;a-Medina B, Estrada-Reyes R, et al. Anxiolytic-like actions of the hexane extract from leaves of Annona cherimolia in two anxiety paradigms: possible involvement of the GABA/benzodiazepine receptor complex. Life Sciences. 2006;78(7):730-737. DOI: https://doi.org/10.1016/j.lfs.2005.05.078Abdou EM, Fayed MAA, Helal D, et al. Assessment of the hepatoprotective effect of developed lipid-polymer hybrid nanoparticles (LPHNPs) encapsulating naturally extracted &beta;-sitosterol against CCl4 induced hepatotoxicity in rats. Scientific Reports. 2019;9(1):19779. DOI: https://doi.org/10.1038/s41598-019-56320-2Park YJ, Bang IJ, Jeong MH, et al. Effects of &beta;-sitosterol from corn silk on TGF-&beta;1-induced epithelial&ndash;mesenchymal transition in lung alveolar epithelial cells. Journal of Agricultural and Food Chemistry. 2019;67(35):9789-9795. DOI: https://doi.org/10.1021/acs.jafc.9b02730Rahman M, Anwar N. Antibacterial and cytotoxic activity of longiverbenone isolated from the rhizome of Cyperus scariosu. Bangladesh Journal of Microbiology. 2008;25(1):82-84. DOI: https://doi.org/10.3329/bjm.v25i1.4866Keawsa-ard S, Liawruangrath B, Liawruangrath S, et al. Chemical constituents and antioxidant and biological activities of the essential oil from leaves of Solanum spirale. Natural Product Communications. 2012;7(7):1934578X1200700. DOI: https://doi.org/10.1177/1934578X1200700740Anwar R, Sukmasari S, Aisyah LS, et al. Antimicrobial activity of &beta;-sitosterol isolated from Kalanchoe tomentosa leaves against Staphylococcus aureus and Klebsiella pneumonia. Pakistan Journal of Biological Sciences. 2022;25(7):602-607. DOI: https://doi.org/10.3923/pjbs.2022.602.607Dahham SS, Tabana YM, Iqbal MA, et al. The anticancer, antioxidant and antimicrobial properties of the sesquiterpene &beta;-caryophyllene from the essential oil of Aquilaria crassna. Molecules. 2015;20(7):11808-11829. DOI: https://doi.org/10.3390/molecules200711808Ganesan T, Subban M, Christopher Leslee DB, et al. Structural characterization of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa and its antioxidant and antibacterial activities. Biomass Conversion and Biorefinery. 2022;14:14547-14558. DOI: https://doi.org/10.1007/s13399-022-03576-wBui TKL, Nguyen TMO, Nguyen TLT, et al. Assessment of antioxidant activities of Curcuma aromatica Salisb extracts. Journal of Science. 2021;18(6):1028-1040.Arivarasu L. In-vitro antioxidant potential of beta-sitosterol: A preface. Cureus. 2023;15(9):e45617. DOI: https://doi.org/10.7759/cureus.45617