<?xml version='1.0' encoding='utf-8'?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd">
<article article-type="research-article" dtd-version="1.2" xml:lang="ru" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><front><journal-meta><journal-id journal-id-type="issn">2658-6533</journal-id><journal-title-group><journal-title>Научные результаты биомедицинских исследований</journal-title></journal-title-group><issn pub-type="epub">2658-6533</issn></journal-meta><article-meta><article-id pub-id-type="doi">10.18413/2313-8955-2015-1-4-62-65</article-id><article-id pub-id-type="publisher-id">498</article-id><article-categories><subj-group subj-group-type="heading"><subject>Архив</subject></subj-group></article-categories><title-group><article-title>МОРФОМЕТРИЧЕСКОЕ ИССЛЕДОВАНИЕ НЕЙРОНОВ ГИППОКАМПА ПРИ ХРОНИЧЕСКОМ ИММОБИЛИЗАЦИОННОМ СТРЕССЕ.</article-title><trans-title-group xml:lang="en"><trans-title>MORPHOMETRIC STUDY OF HIPPOCAMPAL NEURONS IN CHRONIC IMMOBILIZATION STRESS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><name-alternatives><name xml:lang="ru"><surname>Крюков</surname><given-names>Алексей Анатольевич</given-names></name><name xml:lang="en"><surname>Kriukov</surname><given-names>Aleksei A.</given-names></name></name-alternatives><email>KrukovAA@kursksmu.net</email></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="ru"><surname>Тверской</surname><given-names>Алексей Владимирович</given-names></name><name xml:lang="en"><surname>Tverskoi</surname><given-names>Aleksei V.</given-names></name></name-alternatives></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="ru"><surname>Белых</surname><given-names>Андрей Евгеньевич</given-names></name><name xml:lang="en"><surname>Belykh</surname><given-names>Andrey E.</given-names></name></name-alternatives><email>and-white@yandex.ru</email></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="ru"><surname>Должиков</surname><given-names>Александр Анатольевич</given-names></name><name xml:lang="en"><surname>Dolzhikov</surname><given-names>Aleksandr A.</given-names></name></name-alternatives><email>dolzhikov@bsuedu.ru</email></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="ru"><surname>Бобынцев</surname><given-names>Игорь Иванович</given-names></name><name xml:lang="en"><surname>Bobyntsev</surname><given-names>Igor I.</given-names></name></name-alternatives><email>bobig@mail.ru</email></contrib></contrib-group><pub-date pub-type="epub"><year>2015</year></pub-date><volume>1</volume><issue>4</issue><fpage>0</fpage><lpage>0</lpage><self-uri content-type="pdf" xlink:href="/media/medicine/2015/4/med12.pdf" /><abstract xml:lang="ru"><p>Гиппокамп обеспечивает реализацию механизмов памяти, поведенческих реакций, в том числе избегания стрессовых, аверсивных воздействий. Исследование выполнено на материале 20 крыс-самцов Вистар массой 220-250 г, 10 из которых составили интактную контрольную группу, 10 &amp;ndash;экспериментальную, в которой моделировали хронический иммобилизационный стресс. Определяли относительное количество нейронов в нескольких полях зрения на полной площади пирамидного и полиморфного слоев областей СА1 и СА3 (с последующим пересчетом на 10000 мкм), больший и меньший диаметры перикарионов нейронов, их периметры, и площади, диаметры ядер и ядрышек, ядерно-цитоплазматическое соотношение. Установлено, что при хроническом иммобилизационном стрессе в областях СА1 и СА3 гиппокампа наблюдаются морфологически сходные повреждения нейронов, уменьшение их количества, изменение ядерно-цитоплазматического соотношения.</p></abstract><trans-abstract xml:lang="en"><p>Hippocampus ensures the implementation of the memory mechanisms, behavioral reactions, including avoidance of stress, aversive effects etc. The study was performed on the material of 20 male Wistar rats weighing 220-250 g, 10 of which were intact control group and 10 were experimental group, in which chronic immobilization stress was simulated. We determined the relative number of neurons in multiple fields of view on the total area of the pyramidal and polymorphic layers of CA1 and CA3 regions (further recalculated per 10,000 &amp;micro;m), larger and smaller diameters of neuron&amp;rsquo;s bodies, their perimeters and areas with diameters of nuclei and nucleoli, nuclear-cytoplasmic ratio. It is found that under chronic immobilization stress in areas CA1 and CA3 of the hippocampus the morphologically similar neuronal lesions, decrease in their number, and change in nucleocytoplasmic ratio are observed.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>гиппокамп</kwd><kwd>нейроны</kwd><kwd>иммобилизационный стресс</kwd></kwd-group><kwd-group xml:lang="en"><kwd>hippocampus neurons</kwd><kwd>immobilization stress</kwd></kwd-group></article-meta></front><back><ref-list><title>Список литературы</title><ref id="B1"><mixed-citation>1. Умрихин А.Е. Нейромедиаторные гиппокампаль-ные механизмы стрессорного поведения и реакций избегания // Вестник новых медицинских технологий (электронное издание). 2013. № 1. С. 55.</mixed-citation></ref><ref id="B2"><mixed-citation>2. Bartsch T., D&amp;ouml;hring J., Rohr A., Jansen O., Deuschl G. CA1 neurons in the human hippocampus are critical for autobiographical memory, mental time travel, and autonoetic consciousness // PNAS. 2011. Vol. 108, N 42. P. 17562-1756.</mixed-citation></ref><ref id="B3"><mixed-citation>3. Bodnoff S.R., Humphreys A.G., Lehman J.C., Diamond D.M., Rose G.M., Meaney M.J. Enduring effects of chronic corticosterone treatment on spatial learning, synaptic plasticity, and hippocampal neuropathology in young and mid-aged rats // J Neurosci. 1995. Vol. 15. P. 61-69.</mixed-citation></ref><ref id="B4"><mixed-citation>4. Clark R.E., Broadbent N.J., Larry R. Squire Hippocampus and Remote Spatial Memory in Rats // Hippocampus. 2005. 15(2). P. 260 &amp;ndash; 272.</mixed-citation></ref><ref id="B5"><mixed-citation>5. Coburn-Litvak P.S., Tata D.A., Gorby H.E., McCloskey D.P., Richardson G., Anderson B.J. Chronic corticosterone affects brain weight, and mitochondrial, but not glial volume fraction in hippocampal area CA3 // Neuroscience. 2004. Vol. 124, N 2. P. 429-438.</mixed-citation></ref><ref id="B6"><mixed-citation>6. Conrad C.D. Chronic Stress-Induced Hippocampal Vulnerability: The Glucocorticoid Vulnerability Hypothesis// Rev Neurosci. 2008. Vol. 19, N 6. P. 395-411.</mixed-citation></ref><ref id="B7"><mixed-citation>7. Dachir S., Kadar T., Robinzon B., Levy A. Nimodipine&amp;#39;s protection against corticosterone induced morphological changes in the hippocampus of young rats // Brain Res. 1997. Vol. 748, N 1-2. P. 175-183.</mixed-citation></ref><ref id="B8"><mixed-citation>8. El Falougy H., Kubikova E., Benuska J. The microscopical structure of the hippocampus in the rat // Bratisl. Lek Listy. 2008. Vol. 109, N 3. P. 106-110.</mixed-citation></ref><ref id="B9"><mixed-citation>9. Fuchs E., Fl&amp;uuml;gge G., Ohl F., Lucassen P., VollmannHonsdorf G.K., Michaelis T. Psychosocial stress, glucocorticoids, and structural alterations in the tree shrew hippocampus // Physiol Behav. 2001. Vol. 73. P. 285-291.</mixed-citation></ref><ref id="B10"><mixed-citation>10. Leverenz J.B., Wilkinson C.W., Wamble M., Corbin S., Grabber J.E., Raskind M.A., Peskind E.R. Effect of chronic high-dose exogenous cortisol on hippocampal neuronal number in aged nonhuman primates // J Neurosci. 1999. Vol. 19. P. 2356-2361.</mixed-citation></ref><ref id="B11"><mixed-citation>11. M&amp;uuml;ller M.B., Lucassen P.J., Yassouridis A., Hoogendijk W.J.G., Holsboer F., Swaab D.F. Neither major depression nor glucocorticoid treatment affects the cellular integrity of the human hippocampus // Eur J Neurosci. 2001. Vol. 14. &amp;ndash; P. 1603-1612.</mixed-citation></ref><ref id="B12"><mixed-citation>12. Sapolsky R.M., Krey L.C., McEwen B.S. Prolonged glucocorticoid exposure reduces hippocampal neuron number: Implications for aging // J Neurosci. 1985. Vol. 5. P. 1222-1227.</mixed-citation></ref><ref id="B13"><mixed-citation>13. Sapolsky R.M., Uno H., Rebert C.S., Finch C.E. Hippocampal damage associated with prolonged glucocorticoid exposure in primates // J Neurosci. &amp;nbsp;1990.Vol. 10. P. 2897-2902.</mixed-citation></ref><ref id="B14"><mixed-citation>14. Sousa N., Madeira M.D., Paula-Barbosa M.M. Effects of corticosterone treatment and rehabilitation on the hippocampal formation of neonatal and adult rats. An unbiased stereological study // Brain Res. 1998. Vol. 794, N 2. P. 199-210.</mixed-citation></ref><ref id="B15"><mixed-citation>15. The hippocampal book / Edited by: P. Andersen, Morris R., Amaral D., Bliss T., &amp;lsquo;O Keefe J.&amp;nbsp; Oxford University Press. 2007. 832 p.</mixed-citation></ref><ref id="B16"><mixed-citation>&amp;nbsp;</mixed-citation></ref></ref-list></back></article>