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DOI: 10.18413/2658-6533-2022-8-1-0-5

Wound healing effect of organometallic zinc complex in the rat model of planar skin wound
 

Background: The complex and dynamic process of wound healing involves the stages of inflammation, proliferation and maturation of the connective tissue of the scar. Zinc, as an essential trace element, plays an important physiological role at each stage of wound remodeling. Zinc increases the migration and proliferation of keratinocytes, participates in the reepithelization of the epidermis, has anti-inflammatory, antioxidant, immunomodulatory and antimicrobial action. The aim of the study: Comparative study of the effect of the zinc complex of the N-isopropenylimidazole derivative under the code name Pilim-1 on the course of wound uninfected process in modeling of planar skin wound in rats. Materials and methods: 1% gel of Pilim-1 sodium with carboxymethyl cellulose gel as a base has been studied. Methyluracil (10% ointment for topical application) was selected as reference preparation. A model of a "planar skin wound" was reproduced on 24 anesthetized outbred adult male white rats. The degree of healing was evaluated for 28 days by weight by checking the wound area. Wound skin bioptats were taken on day 29 after the start of treatment. On histological sections, the presence of capillaries, fibroblasts, epitheliocytes, white blood cells, the degree of severity of granulation tissue and its maturity, presence of necrosis and inflammation were evaluated. Results: Local application of 1% Pilim-1 gel was found to reduce the healing time of wounds compared to the control group of animals.  In animals treated with Pilim-1, complete wound regeneration was noted on the 24th day. On days 10, 21, 24, 29, a statistically significant decrease in the size of the wound was revealed in this group compared to the control group. In the group of animals treated with methyluracil, complete wound healing was observed only in 3 rats on the 29th and 24th days of observation. On histological sections, signs of complete epithelization, restoration of skin epithelialization and the presence of mature granulation tissue were noted. Conclusion: The identified regenerative effect of the zinc complex is of interest for further study in order to develop an effective agent that stimulates reparative regeneration of wounds.

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