DOI: 10.18413/2313-8955-2018-4-1-70-76

STUDY OF POLYSACCHARIDES OF THE ACROPTILON REPENS L. HERB OF THE FLORA OF TULA REGION

The aim of the research was to study the component composition of polysaccharide complexes of the Acroptilon repens L. herb. Materials and methods. The air-dry Acroptilon repens L. herb, harvested in 2017 on the territory of the Tula region in the period of mass flowering was used as an object of the research. Isolation of polysaccharide complexes was carried out fractionally, according to the method of Kochetkov (first, there were obtained water-soluble polysaccharide complexes, and then – pectin substances and hemicelluloses A and B). The monosaccharide composition of the isolated polysaccharide complexes was established by acid hydrolysis, followed by chromatography with n-butanol – pyridine – water solvent systems purified (6 : 4 : 3) and ethylacetate – acetic acid – formic acid – purified water (18 : 3 : 1 : 4 ) in parallel with reliable samples. The quantitative content of sugars in the hydrolysates of polysaccharides was determined by densitometry. Titrometric methods were used for the quantitative determination of functional groups in the pectin substances of the investigated raw material. Results of the study. In the course of the study, water-soluble polysaccharide complexes, pectin substances, hemicelluloses A and B were isolated from the Acroptilon repens L. herb. It was established that pectic substances (12.6%) and water-soluble polysaccharides (4.03%) were the predominant substances. In water-soluble polysaccharides of the Acroptilon repens L. herb, arabinose (7.7%) and galactose (6.9%) predominate. The basis of pectin substances is galacturonic acid (83.6%). In hemicelluloses, xylose is the predominant monosaccharide. The pectin substances of the Acroptilon repens L. herb are characterized by a low (<50%) degree of esterification.

Keywords: Acroptilon repens L, Asteraceae, polysaccharides.

Number of views: 3220 (view statistics)

Количество скачиваний: 5067

Full text (HTML)Full text (PDF)To articles list

Information for citation:

Shestopalova NN. Study of polysaccharides of the Acroptilon repens L. herb of the flora of Tula region. Research Result. Medicine and Pharmacy. 2018;4(1):70-76 (In Russian). DOI: 10.18413/2313-8955-2018-4-1-70-76

User comments
Reference lists

While nobody left any comments to this publication.
You can be first.

Boytsova, T.M., Nazarova, O.M. (2015), “Obosnovaniye usloviy ekstraktsii polisakharidov iz nastoya semeni lna” [Justification of the conditions for extraction of polysaccharides from the infusion of flax seed], Fundamentalnyye issledovaniya, 8-1, 14-18. Russian.
Bubenchikova, V.N., Stepnova, I.V., Vorobyeva, E.A. (2016), “Izucheniye veshchestv pervichnogo biosinteza travy gorlyukhi yastrebinkovoy (Picris hieracioides L.)” [The study of substances of primary biosynthesis of hawkweed oxtongue (Picris hieracioides L.)], Meditsinskiy vestnik Bashkortostana, 11, 5(65), 67-70. Russian.
Krishtanova, N.A., Safonova, M.Yu., Bolotova, V.Ts. i dr. (2005), “Perspektivy ispolzovaniya rastitelnykh polisakharidov v kachestve lechebnykh i lechebno-profilakticheskikh sredstv” [Prospects for the use of plant polysaccharides as therapeutic and therapeutic-prophylactic means], Vestnik VGU. Seriya: Khimiya. Biologiya. Farmatsiya, 1, 212-221. Russian.
Malyutina, A.Yu., Shestopalova, N.N. (2015), “Issledovaniye sostava polisakharidnykh kompleksov Achyrophorus maculatus L. flory Tulskoy oblasti” [Investigation of the Achyrophorus maculatus L. polysaccharide complex composition of the flora of Tula region], Nauchnyy rezultat. Seriya Meditsina i Farmatsiya, 1, 3, 143-150. Russian.
Safonova, E.A., Lopatina, K.A., Razina, T.G., Fedorova, E.P., Zuyeva, E.P., Guryev, A.M., Belousov, M.V. (2017), “Eritropoezindutsiruyushchaya aktivnost polisakharidov Tussilago farfara L. na fone kombinirovannogo primeneniya tsisplatina i etopozida” [Erythropoiesis-induced activity of polysaccharides of Tussilago farfara L. against the background of the application of cisplatin and etoposide], Sibirskiy onkologicheskiy zhurnal, 16(4), 42-48. Russian.
Tkachenko, A.N., Kulikova, M.D., Malyutina, A.Yu. (2016), “Acroptilon repens L. – perspektivnoye rasteniye narodnoy meditsiny” [Acroptilon repens L. as a promising plant of traditional medicine], Vzglyad budushchikh spetsialistov na problemy sovremennoy meditsiny: sbornik tezisov nauchnoy sessii meditsinskogo instituta NIU «BelGU» / pod obshch. red. O.A. Efremovoy. Belgorod: ID «Belgorod» NIU «BelGU», 112. Russian.
Bacáková, L., Novotná, K., Parízek, M. (2014), “Polysaccharides as cell carriers for tissue engineering: the use of cellulose in vascular wall reconstruction”, Physiological Research, 63 (1), 29-47.
Kim, K.J., Lee, O.H., Lee, B.Y. (2010), “Fucoidan, a sulfated polysaccharide, inhibits adipogenesis through the mitogen-activated protein kinase pathway in 3T3-L1 preadipocytes”, Life Sci, 86(21-22), 791-797.
Kumar, D., Pandey, J., Raj, V., Kumar, P. (2017), “A review on the modification of polysaccharide through graft copolymerization for various potential applications”, The Open Medicinal Chemistry Journal, 11, 109-126.
Laurienzo, P., Fernandes, J.C., Colliec-Jouauland, J., Fitton, S. (2015), “The use of natural polysaccharides as biomaterials”, BioMed Research International, 1-2.
Li, Ch., Li, X., You, L., Fu, X., Liu, R.H. (2017), “Fractionation, preliminary structural characterization and bioactivities of polysaccharides from Sargassum pallidum”, Carbohydrate Polymers, 155, 261-270.
Liu, J., Du, C., Wang, Y., Yu, Zh. (2015), “Anti-fatigue activities of polysaccharides extracted from Hericium erinaceus”, Experimental and Therapeutic Medicine, 9(2), 483-487.
Wang, P.-Ch., Zhao, Sh., Yang, B.-Y., Wang, Q.-H., Kuang, H.-X. (2016), “Anti-diabetic polysaccharides from natural sources: a review”, Carbohydrate Polymers, 148, 86-97.
Zheng, Ya., Wang, Q., Lu, X., Zheng, B., Xiao, J. (2016), “Antitumor and immunomodulatory activities of the hot water-soluble polysaccharides from lotus (Nelumbo nucifera Gaertn.) seeds”, Free Radical Biology and Medicine, 100, 133.

All journals

Send article

Research Results in Biomedicine is included in the scientific database of the RINTs (license agreement No. 765-12/2014 dated 08.12.2014).

The journal is included in the list of peer-reviewed scientific publications recommended by the Higher Attestation Commission

The journal is indexed by the following scientific databases and platforms

Research Results in Biomedicine (ISSN 2658-6533)

The journal materials and website are licensed under Creative Commons «Attribution» 4.0 International.

The Founder: Federal State Autonomous Educational Institution of Higher Education "Belgorod National Research University"The Founder’s address: 85 Pobedy Street, Belgorod, the Belgorod region, 308015, Russia

The Publisher: Federal State Autonomous Educational Institution of HigherEducation "Belgorod National Research University" The Founder’s address:85 Pobedy Street, Belgorod, the Belgorod region, 308015, Russia

Editors Office: chief editor Mikhail I. Churnosov, e-mail: RR_MedPharm@bsu.edu.ru, phone: +7 (4722) 30-14-03.

Regulations on the Journal

Certificate

Have questions?
You can write to us:

✉ Content manager

✉ Site administration

✉ Executive Secretary