Association between mitochondrial DNA copy number and high viral load in women with high-risk human papillomavirus
Background: The infection that most often leads to cervical cancer is human papillomavirus (HPV) infection. A sign that the HPV infection might develop into cancer is mitochondrial dysfunction and DNA damage. Increased mitochondrial DNA copy number (mtCN) has been associated with cervical neoplasm as a compensatory mechanism for mitochondrial dysfunction. The aim of the study: To compare the variation of the mitochondrial DNA levels in the women's samples with high HPV load and the control group. Materials and methods: An investigation was carried out using a sample of 100 women. Half of the sample had been diagnosed with HPV. The HPV-DNA was analyzed using an Amplisens HPV HCR screen-titre-FRT PCR kit, while real-time PCR was employed to ascertain the relative mitochondrial DNA copy number content. Results: A statistical analysis found no correlation between mtCN and age for both sample cases and controls (r = - 0.11 and p = 0.44; r = 0.053, p = 0.71, respectively). A high level of infection was associated with a higher relative mitochondrial DNA content, in comparison with levels observed in the sample of healthy women (25.78 versus 18.13, respectively). Conclusion: This study significantly enhances the evidence that the mtCN is linked to an increased risk of high HPV load.
AlBosale AHH, Mashkina EV. Association between mitochondrial DNA copy number and high viral load in women with high-risk human papillomavirus. Research Results in Biomedicine. 2023;9(2):173-180. DOI: 10.18413/2658-6533-2023-9-2-0-2
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