Morphological changes in gum tissue during thiram intoxication followed by correction with resveratrol and sea buckthorn oil
Background: The widespread use of pesticides can lead to a violation of the ecological balance between the environment and the human body, provoking the development of oxidative stress, which leads to an increase in toxic forms of damage to organs and systems of the macroorganism. In order to suppress oxidative stress that develops in many pathological processes, it is advisable to use antioxidants that inhibit the formation of free radicals. The aim of the study:To study the morphological changes in gum tissues in case of thiram intoxication followed by correction with resveratrol and sea buckthorn oil. Materials and methods: The experiment was performed on 50 rats that received the fungicide thiram for 28 days, followed by correction with resveratrol or sea buckthorn oil. To assess the severity of inflammatory changes in histological preparations, morphofunctional changes in the mucosal epithelium and the cellular composition of the subepithelial layer were analysed. Fibroblastic differon cells, macrophages and inflammatory cells of granulocytes and lymphocytes were differentiated by karyological signs, and the thickness of the epithelial layer, the area occupied by the fibrous, vascular and cellular components were measured. Results: In the experimental groups “Thiram 28 days” and “Thiram+standard diet” in all rats, the development of an inflammatory reaction of the gum tissue was observed, manifested in hyperkeratosis and an increase in the thickness of the epithelium up to 173.438 microns and 230.568 microns, diffuse polymorphocellular infiltration of the subepithelial layer mainly due to granulocytes to 21.40 and 21.92, respectively, expansion of the lumen and blood filling of the vessels of the microvasculature. After the use of resveratrol and sea buckthorn oil, the thickness of the epithelial layer was restored to 114,07 and 190,03 microns, respectively, interstitial edema and cell infiltration of the subepithelial layer decreased. At the same time, due to migration and increase of fibroblastic differon cells (28.96 and 29.44), neocollagenogenesis processes and an increase in the fibrous component were observed up to 65.62 and 33.69 after application of resveratrol and sea buckthorn oil, respectively. Conclusion: The effectiveness of monotherapy with resveratrol and sea buckthorn oil in normalizing the morphology of gum tissue has been proven. At the same time, resveratrol has both an anti-inflammatory effect and a high regenerative effect
Korolev VA, Mishina ES, Felker EV, et al. Morphological changes in gum tissue during
thiram intoxication followed by correction with resveratrol and sea buckthorn oil. Research Results
in Biomedicine. 2024;10(1):99-111. Russian. DOI: 10.18413/2658-6533-2024-10-1-0-6
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