Status of the glutathione antioxidant defense in fungicide intoxication and correction with vitamin A and milk thistle
Background: Thiram entering with food products into tissues and organs of animals and humans can lead to the activation of free-radical oxidation processes and, as a consequence, imbalance in the functioning of the antioxidant defense system of the body. The aim of the study: Determination of the state of the glutathione unit of the antioxidant defense of the body during fungicidal intoxication and correction with vitamin A and milk thistle. Materials and methods: Subchronic intoxication with thiram at a dose of 1/50 LD50 (1.6 mg) was carried out for 4 weeks under in vivo exposure to 240 Wistar rats at the age of 2 months. The effect of thiram on the indicators of the antioxidant defense of the body was studied using the example of the variability of the catalytic activity of glutathione peroxidase (GPX), glutathione reductase (GSR), the quantitative content of reactive oxygen species (ROS) and glutathione subfractions (GSH/GSSG). Results: In this publication, the effect of thiram intoxication on the antioxidant defense parameters on the body of white Wistar rats' antioxidant defense parameters using the sample of the variability of the catalytic activity of glutathione peroxidase (GPX), glutathione reductase (GSR), the quantitative content of reactive oxygen species (ROS) and glutathione subfractions (GSH/GSSG). The modeling of subchronic intoxication was accompanied by a significant increase in the content of ROS, an increase in the activity of GPX, GSR (p<0.05). At the same time there is a significant decrease in the GSH/GSSG ratio in the blood plasma and oral fluid of rats in comparison with the control group. After the experimental subchronic intoxication some antioxidants were used, such as vitamin A (retinol acetate) and milk thistle. Their usage for 30 days has led to the restoration of the studied parameters. Conclusion: Thus, we have shown that an increase in the formation of free radicals and an imbalance in the functioning of the glutathione pool of antioxidant protection occurs due to the intake of micro-amounts of the fungicide thiram into the body. The use of vitamin A and milk thistle helps to restore the prooxidant-antioxidant balance of the body. The results obtained can be used in antioxidant therapy to stop the processes of oxidative stress in pesticide intoxication.
Korolev VA, Sedykh AV, Azarova YuE, et al. Status of the glutathione antioxidant defense in fungicide intoxication and correction with vitamin A and milk thistle. Research Results in Biomedicine. 2022;8(2): 207-220. Russian. DOI:10.18413/2658-6533-2022-8-2-0-6
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