State of colon parietal microbiota and antioxidant properties of colonocytes in rats with ecological dysbiosis treated with sea vitamin E and buckthorn oil
Background: When the macroorganism is exposed to pesticides, qualitative and/or quantitative changes in the microbiocenosis composition occur – dysbiosis. As a result of this action, lipid peroxidation is enhanced and work of the body's antioxidant system is disrupted. The aim of the study: To study the state of colon microbiota and antioxidant properties of colonocytes in rats with subchronic thiram intoxication treated with vitamin E and sea buckthorn oil. Materials and methods: Animals in the experiment received thiram. Vitamin E and sea buckthorn oil were used as antioxidants. The composition of rat colon mucosal microflora was judged. The activity of antioxidant protection enzymes of colonocytes was studied by content of superoxide dismutase and catalase. The state of lipid peroxidation was assessed by content of diene conjugates and malondialdehyde. Results: The administration of thiram led to a decrease in the number of Bifidobacteria by 3 times (p<0.001), Lactobacillus by 2.5 times (p<0.001), E. coli with normal enzymatic activity by 2.2 times (p<0.001), Escherichia with reduced enzymatic activity by 2.8 times (p<0.001), Enterobacter by 1.8 times (p<0.001), Salmonella by 1.7 times (p<0.01), Citrobacter by 1.75 times (p<0.01), coagulase-negative Staphylococci by 2.1 times (p<0.001), Enterococci by 1.5 times (p<0.05). There was a decrease in the activity of catalase by 1.9 times (p<0.001), superoxide dismutase by 2.3 times (p<0.001) and an increase in the concentration of malondialdehyde by 2.6 times (p<0.001), diene conjugates by 2.4 times (p<0.001). The use of vitamin E contributed to an increase in the number of Lactobacillus by 1.4 times (p<0.05), a decrease in the number of Enterobacter, Morganella and Acinetobacter by 1.5 times (p<0.05), 2 times (p<0.001) and 1.8 times (p<0.01), respectively. A decrease in the content of malondialdehyde by 3 times (p<0.001), diene conjugates by 2.4 times (p<0.001) and an increase in the activity of catalase by 1.5 times (p<0.01), and superoxide dismutase by 1.6 times (p<0.001) was found. The use of sea buckthorn oil led to a decrease in the number of Enterobacter by 1.6 times (p<0.001), Morganella by 2.3 times (p<0.001), and Acinetobacter by 2 times (p<0.001). An increase in the activity of catalase by 1.4 times (p<0.05), superoxide dismutase by 1.6 times (p<0.001) and a decrease in the concentration of malondialdehyde by 2.9 times (p<0.001), diene conjugates by 1.9 times (p<0.001). Conclusion: The use of E and sea buckthorn oil increase protective properties of body during experimental dysbiosis.
Korolev VA, Medvedeva OA, Riadnova VA, et al. State of colon parietal microbiota and antioxidant properties of colonocytes in rats with ecological dysbiosis treated with sea vitamin E and buckthorn oil. Research Results in Biomedicine. 2023;9(1):71-85. Russian. DOI: 10.18413/2658-6533-2023-9-1-0-5
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