This paper presents an original method for the analysis of corticosteroids with the use of reversed-phase high performance liquid chromatography. A mixture of 1% aqueous formic acid and ethyl alcohol at a ratio of 10 to 90 was taken as an eluting system for corticosteroids. This ratio of solvents in the mobile phase ensures control of the rate of movement of corticosteroids in the column. Since corticosteroids are freely soluble in alcohol, using 90% of this solvent in the mobile phase ensured optimum retention time of these compounds. Another important advantage of this system is that the expensive and toxic acetonitrile and methanol, commonly used in the world practice in the reversed-phase HPLC were replaced with non-toxic and available ethyl alcohol. The quality of the chromatographic results remained the same. Due to characteristic UV and visible spectrum absorption bands of the corticosteroids, we used the diode-array detector to record the results. Analysis time was maximum 5 minutes, which characterizes the chromatographic process as express enough.