Association of the ITGB3 gene T1565C polymorphism with the development of atherosclerosis and in-stent restenosis in patients with stable coronary artery disease
Background: Today, percutaneous coronary intervention (PCI) is the most effective treatment of coronary artery disease (CAD). Despite all technical advances in stent designs and techniques, in-stent restenosis (ISR) remains one of the main limiting factors of this procedure. Studies in the field of molecular cardiology have shown a significant contribution of endothelial dysfunction in the development of ISR. According to the literature, integrin beta 3 is involved in intercellular interactions, interaction with extracellular matrix; it influences the activity of smooth muscle cells, which can lead to neointimal hyperplasia. The aim of the study: To study the possibility of association of T1565C polymorphism of the ITGB3 gene with the development of restenosis after stenting of the coronary arteries. Materials and methods: Patients with CAD after PCI with drug-coated stents (n = 110) and patients with intact vessels according to angiography (n = 62) were included in the study. The repeated stenosis of the artery at the stent implantation site of more than 50% was defined as angiographic restenosis. The criteria for inclusion in the study were: age > 45 years, ethnic Russians, atherosclerosis according to the angiography of one or more arteries, patient informed consent. The genotyping for the ITGB3 T1565C polymorphism was performed using the real-time PCR. Results: The C allele and heterozygous genotype frequencies are significantly higher in groups of patients with earlier development of restenosis and diffuse CAD with occlusion. Conclusion: The minor C allele of the ITGB3 T1565C polymorphism may be considered as a predictor of diffuse CAD with the development of occlusions and ISR of the coronary arteries within the first year after stent implantation.