DOI: 10.18413/2658-6533-2019-5-3-0-1

The applicability of interphase chromosome-specific multicolor banding (ICS-MCB) for studying neurodevelopmental and neurodegenerative disorders

Ivan Y. Iourov
Thomas Liehr
Svetlana G. Vorsanova
Luis A. Méndez-Rosado
Yuri B. Yurov

Background: Interphase chromosome-specific multicolor banding (ICS-MCB) has been developed for studying whole chromosomes in interphase nuclei at any stage of the cell cycle at molecular resolution. Previously, important biomedical discoveries have been made using the technique. In the postgenomic era, a need appears to exist for a reevaluation of molecular cytogenetic techniques, including ICS-MCB, which seems to take a well-deserved place. Aim of the study: The aim of the present study is to address the applicability of ICS-MCB for studying neurodevelopmental and neurodegenerative disorders. Conclusions: A brief overview of previous ICS-MCB applications demonstrates that the technique may provide an appreciable amount of unique data on chromosome abnormalities and organization in interphase nuclei. Furthermore, the technique offers opportunities for evaluating these phenomena in the diseased human brain. Such opportunity seems to be critical for unraveling molecular and cellular mechanisms of neurodevelopmental and neurodegenerative disorders. Therefore, we conclude that ICS-MCB may represent an important part of molecular and cellular studies of neurodevelopmental and neurodegenerative disorders.

Keywords: chromosome, chromosome instability, interphase nucleus, neurodegenerative disorders, neurodevelopmental disorders.

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Iourov IY, Liehr T, Vorsanova SG, et al. The applicability of inter-phase chromosome-specific multicolor banding (ICS-MCB) for studying neurode-velopmental and neurodegenerative disorders. Research Results in Biomedicine. 2019;5(3):4-9. DOI: 10.18413/2658-6533-2019-5-3-0-1

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Iourov IY, Vorsanova SG, Yurov YB. Molecular cytogenetics and cytogenomics of brain diseases. Curr Genomics. 2008;9(7):452-465. DOI: https://doi.org/10.2174/138920208786241216
Parikshak NN, Gandal MJ, Geschwind DH. Systems biology and gene networks in neurodevelopmental and neurodegenerative disorders. Nat Rev Genet. 2015;16(8):441-458. DOI: https://doi.org/10.1038/nrg3934
Yurov YB, Vorsanova SG, Iourov IY. Human Molecular Neurocytogenetics. Curr Genet Med Rep. 2018;6(4):155-164. DOI: https://doi.org/10.1007/s40142-018-0152-y
Willsey AJ, Morris MT, Wang S, et al. The psychiatric cell map initiative: a convergent systems biological approach to illuminating key molecular pathways in neuropsychiatric disorders. Cell. 2018;174(3):505-520. DOI: https://doi.org/10.1016/j.cell.2018.06.016
Youssoufian H, Pyeritz RE. Human genetics and disease: Mechanisms and consequences of somatic mosaicism in humans. Nat Rev Genet. 2002;3(10):748-758. DOI: https://doi.org/10.1038/nrg906
Rohrback S, Siddoway B, Liu CS, et al. Genomic mosaicism in the developing and adult brain. DevNeurobiol. 2018;78(11):1026-1048. DOI: https://doi.org/10.1002/dneu.22626
Iourov IY, Vorsanova SG, Yurov YB, et al. Ontogenetic and pathogenetic views on somatic chromosomal mosaicism. Genes (Basel). 2019;10(5). pii: E379. DOI: https://doi.org/10.3390/genes10050379
Speicher MR, Carter NP. The new cytogenetics: blurring the boundaries with molecular biology. Nat Rev Genet. 2005;6(10):782-792. DOI: https://doi.org/10.1038/nrg1692
Bakker B, van den Bos H, Lansdorp PM, et al. How to count chromosomes in a cell: An overview of current and novel technologies. BioEssays, 2015;37(5):570-577. DOI: https://doi.org/10.1002/bies.201400218
Vorsanova SG, Yurov YB, Iourov IY. Human interphase chromosomes: a review of available molecular cytogenetic technologies. MolCytogenet. 2010;3:1. DOI: https://doi.org/10.1186/1755-8166-3-1.
Levsky JM, Singer RH. Fluorescence in situ hybridization: past, present and future. J Cell Sci, 2003;116(Pt14):2833-2838. DOI: https://doi.org/10.1242/jcs.00633
Yurov YB, Vorsanova SG, Iourov IY, et al. Unexplained autism is frequently associated with low-level mosaic aneuploidy. J Med Genet. 2007;44(8):521-525. DOI: https://doi.org/ https://jmg.bmj.com/content/44/8/521
Ferguson-Smith MA. History and evolution of cytogenetics. MolCytogenet. 2015;8:19. DOI: https://doi.org/10.1186/s13039-015-0125-8
Cheng L, Zhang S, Wang L, et al. Fluorescence in situ hybridization in surgical pathology: principles and applications. J PatholClin Res. 2017;3(2):73-99. DOI: https://doi.org/10.1002/cjp2.64
Ratan ZA, Zaman SB, Mehta V, et al. Application of fluorescence in situ hybridization (FISH) technique for the detection of genetic aberration in medical science. Cureus. 2017;9(6):e1325. DOI: https://doi.org/10.7759/cureus.1325
Iourov IY, Liehr T, Vorsanova SG, et al. Visualization of interphase chromosomes in postmitotic cells of the human brain by multicolour banding (MCB). Chromosome Res. 2006;14(3):223-229. DOI: https://doi.org/10.1007/s10577-006-1037-6
Iourov IY, Liehr T, Vorsanova SG, et al. Interphase chromosome-specific multicolor banding (ICS-MCB): a new tool for analysis of interphase chromosomes in their integrity. Biomol Eng. 2007;24(4):415-417. DOI: https://doi.org/10.1016/j.bioeng.2007.05.003
Riegel M. Human molecular cytogenetics: from cells to nucleotides. Genet Mol Biol. 2014;37(Suppl1):194-209.
Arendt T, Mosch B, Morawski M. Neuronal aneuploidy in health and disease: a cytomic approach to understand the molecular individuality of neurons. Int J Mol Sci. 2009;10(4):1609-1627. DOI: https://doi.org/10.3390/ijms10041609
Hochstenbach R, Buizer-Voskamp JE, Vorstman JA, et al. Genome arrays for the detection of copy number variations in idiopathic mental retardation, idiopathic generalized epilepsy and neuropsychiatric disorders: lessons for diagnostic workflow and research. Cytogenet Genome Res. 2011;135(3-4):174-202. DOI: https://doi.org/10.1159/000332928
Yurov YB, VorsanovaSG, Solov’ev IV, et al. Instability of chromosomes in human nerve cells (normal and with neuromental diseases). Russ. J. Genet. 2010;46(10):1194-1196. DOI:
Iourov IY, Vorsanova SG, Liehr T, et al. Increased chromosome instability dramatically disrupts neural genome integrity and mediates cerebellar degeneration in the ataxia-telangiectasia brain. Hum Mol Genet. 2009;18(14):2656-69. DOI: https://doi.org/10.1093/hmg/ddp207
Leija-Salazar M, Piette C, Proukakis C. Review: Somatic mutations in neurodegeneration. NeuropatholApplNeurobiol. 2018;44(3):267-285. DOI: https://doi.org/10.1111/nan.12465
Iourov IY, Vorsanova SG, Liehr T, et al. Aneuploidy in the normal, Alzheimer's disease and ataxia-telangiectasia brain: differential expression and pathological meaning. Neurobiol Dis. 2009;34(2):212-220. DOI: https://doi.org/10.1016/j.nbd.2009.01.003
Iourov IY, Vorsanova SG, Yurov YB. Genomic landscape of the Alzheimer's disease brain: chromosome instability – aneuploidy, but not tetraploidy – mediates neurodegeneration. Neurodegener Dis. 2011;8(1-2):35-37. DOI: https://doi.org/10.1159/000315398
Yurov YB, Vorsanova SG, Liehr T, et al. X chromosome aneuploidy in the Alzheimer's disease brain. MolCytogenet. 2014;7(1):20. DOI: https://doi.org/10.1186/1755-8166-7-20
Nudelman KNH, McDonald BC, Lahiri DK, et al. Biological Hallmarks of Cancer in Alzheimer's Disease. MolNeurobiol. 2019:15. DOI: https://doi.org/10.1007/s12035-019-1591-5
Yurov YB, Vorsanova SG, Iourov IY. Ontogenetic variation of the human genome. Curr Genomics. 2010;11(6):420-425. DOI: https://doi.org/ 10.2174/138920210793175958
Zhang L, Vijg J. Somatic mutagenesis in mammals and its implications for human disease and aging. Annu Rev Genet. 2018;52:397-419. DOI: https://doi.org/10.1146/annurev-genet-120417-031501
Iourov I, Vorsanova S, Liehr T, et al. Chromothripsis as a mechanism driving genomic instability mediating brain diseases. MolCytogenet. 2017;10(Suppl 1):20.
Horne SD, Chowdhury SK, Heng HH. Stress, genomic adaptation, and the evolutionary trade-off. Front. Genet. 2014;5:92. DOI: https://doi.org/10.3389/fgene.2014.00092
Hou Y, Song H, Croteau DL, et al. Genome instability in Alzheimer disease. Mech Ageing Dev. 2017;161(PtA):83-94. DOI: https://doi.org/10.1016/j.mad.2016.04.005
Iourov IY, Vorsanova SG, Yurov YB. Somatic cell genomics of brain disorders: a new opportunity to clarify genetic-environmental interactions. Cytogenet Genome Res. 2013;139(3):181-188. DOI: https://doi.org/10.1159/000347053

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