Study of associations of haplotypes of FLG gene polymorphism with the development of chronic true eczema in men
Background: Eczema is a common chronic disease characterized by inflammation of the skin. The aim of the study: To analyze the involvement of haplotypes of polymorphic loci of the filaggrin gene (FLG) in the development of chronic true eczema in men. Materials and methods: The sample for the study included 203 men, including 113 patients with chronic true eczema and 90 men without this pathology. Genotyping of 10 polymorphic loci of the FLG gene was performed. The haploview V. 4.2 program was used to analyze the coupling disequilibrium between SNPs and to construct haploblocks. In accordance with the algorithms "Solid Spine" and "Four gamete frequencies" with a specified threshold D'>0.8, the block structure was determined. The calculation of haplotype frequencies and analysis of their associations with the formation of the disease was performed using the PLINK V. 2.050 software using the EM algorithm. Results: 6 haploblocks were found both in the combined sample of men with chronic true eczema and control, and separately in the groups of patients and control men. There were no significant associations of haplotypes in the six haploblocks considered with the occurrence of chronic true eczema in men. Analysis of the involvement of haplotypes of polymorphic loci of the FLG gene, which are in non-equilibrium by coupling, showed a significant association of the GG rs61816761-rs3126085 haplotype with chronic true eczema in men (OR=0.47, p=0.035, ррerm=0.045). Conclusion: The GG haplotype of the rs61816761-rs3126085 polymorphic loci of the FLG gene is involved in the development of chronic true eczema in men.